Systematic Tuning the Hydrodynamic Diameter of Uniformed Fluorescent Silica Nanoparticles
Systematic Tuning the Hydrodynamic Diameter of Uniformed Fluorescent Silica Nanoparticles
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DOI：
10.1021/jp204323d
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发表时间：
2011-08-25
影响因子：
3.7
通讯作者：
Ozcelik, Serdar
中科院分区：
文献类型：
--
作者：
Durgun, Gulay;Ocakoglu, Kasim;Ozcelik, Serdar
我们报告了一种简单的方法来系统地调整尺寸范围从12到465纳米的均匀荧光二氧化硅颗粒的水动力直径。动态光散射和电子显微镜研究表明，二氧化硅颗粒的水动力尺寸分布是均匀的。我们表明，乙醇和氨的初始量对调整这些颗粒的大小至关重要。这种颗粒的水动力直径随着氨含量的增加而增加。乙醇含量的增加会导致更小颗粒的形成。初始乙醇量越高，乙醇离子浓度越高，氢氧化物离子浓度越低。氢氧根离子的浓度是形成硅氧烷键的原因，这种对氢氧根离子浓度的控制使二氧化硅颗粒的生长受到控制，从而导致流体动力学尺寸的精确调整。我们证实了颗粒的大小和亮度之间存在线性关系，表明这种颗粒中的染料分子的数量可以通过所提出的方法进行调节。我们证明了二氧化硅网络为包裹在颗粒中的染料分子提供了保护，使其免受溶剂、荧光猝灭剂和不利pH环境的影响。此外，12、50和250 nm大小的荧光二氧化硅颗粒对MCF7和PC3上皮细胞系没有细胞毒性，即使剂量达到1.0 mg/mL，孵育时间达到72 h。
We report a facile method for systematic tuning of the hydrodynamic diameter of uniformed fluorescent silica particles in the size range from 12 to 465 nm. Dynamic light scattering and electron microscopy studies demonstrate that the hydrodynamic size distribution of the silica particles is uniform. ' We show that the initial amount of ethanol and ammonia are essential to tune the size of these particles. The hydrodynamic diameter of such a particle increases as the amount of ammonia is increased. An increase in the amount of ethanol leads to the formation of smaller particles. A higher initial amount of ethanol yields an increase in the concentration of ethoxide ions and a decrease in the concentration of hydroxide ions. Such control over the concentration of hydroxide ion, which is responsible for the formation of siloxane bonds, causes a controlled-growth of the silica particles, resulting in precise tuning of the hydrodynamic size. We confirm that a linear relationship exists between the size and brightness of particles, demonstrating that the amount of dye molecules in such particles can be regulated by the presented method. We prove that the silica network provides protection for dye molecules encapsulated in particles against solvents, fluorescence quenchers, and unfavorable pH of environments. Moreover, the fluorescent silica particles with the size of 12, 50, and 250 nm were found to not be cytotoxic against the epithelial cell lines of MCF7 and PC3, even when the dosage levels up to 1.0 mg/mL and incubation periods up to 72 h were applied.