Evaluation of the transdermal permeation of different paraben combinations through a pig ear skin model
Evaluation of the transdermal permeation of different paraben combinations through a pig ear skin model
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DOI：
10.1016/j.ijpharm.2010.02.006
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发表时间：
2010-05-31
影响因子：
5.8
通讯作者：
Oliveira Simoes, Claudia Maria
中科院分区：
文献类型：
--
作者：
Caon, Thiago;Oliveira Costa, Ana Carolina;Oliveira Simoes, Claudia Maria
虽然对羟基苯甲酸酯具有理想防腐剂的几个特征，但不同的研究表明，由于其雌激素活性，它们可能会影响人体健康。因此，已经应用了各种策略来减少它们的皮肤渗透。然而，尚未研究对羟基苯甲酸酯组合对经皮渗透的影响。因此，本研究的目的是使用Franz扩散池系统和毛细管电泳检测评价对羟基苯甲酸酯在猪耳皮肤中的渗透，以确定哪些对羟基苯甲酸酯组合（通过析因设计定义）具有最低的皮肤渗透。还评价了分离的对羟基苯甲酸酯的渗透性，通过Moser模型获得的渗透特性证实亲脂性和分子量可能影响这些化合物的全身吸收。在先前使用分离的对羟基苯甲酸酯的测试中，与真皮相比，对羟基苯甲酸甲酯和对羟基苯甲酸乙酯在表皮中呈现更大的保留，而对羟基苯甲酸丙酯和对羟基苯甲酸丁酯在这些层中具有相似的保留曲线。与表皮相比，乙醇浓度和实验时间的增加促进了对羟基苯甲酸酯在真皮中的更大保留。对羟基苯甲酸甲酯和对羟基苯甲酸乙酯以及对羟基苯甲酸甲酯和对羟基苯甲酸丙酯的二元组合（添加到几种化妆品中以增加抗微生物谱）显著降低了它们通过猪耳皮肤的渗透速率（EP除外），这可能是由于这些对羟基苯甲酸酯在表皮和真皮中的高保留。(C)2010 Elsevier B.V.保留所有权利。
Although parabens have several features of ideal preservatives, different studies have shown that they may affect human health due to their estrogenic activity. Therefore, various strategies have been applied to reduce their skin penetration. However, the effect of paraben combinations on transdermal permeation has not yet been investigated. Thus, the objective of this study was to evaluate paraben permeation in pig ear skin using a Franz diffusion cell system with capillary electrophoresis detection, in order to identify which paraben combinations (defined by a factorial design) have the lowest skin permeation. The permeation of isolated parabens was also evaluated and the permeation characteristics, obtained by the Moser model, confirmed that lipophilicity and molecular weight may influence the systemic absorption of these compounds. In previous tests using isolated parabens, methyl and ethyl parabens presented greater retention in the epidermis compared to the dermis, while propyl and butyl parabens had similar retention profiles in these layers. An increase in ethanol concentration and experimental time promoted greater parabens retention in the dermis compared to the epidermis. The binary combinations of methyl and ethyl parabens as well as of methyl and propyl parabens (added to several cosmetic products in order to increase the antimicrobial spectrum) reduced significantly their permeation rates through pig ear skin (with the exception of EP), probably due to the high retention of these parabens in the epidermis and dermis. (C) 2010 Elsevier B.V. All rights reserved.