Phenotypic and molecular evolution across 10,000 generations in laboratory budding yeast populations.
Phenotypic and molecular evolution across 10,000 generations in laboratory budding yeast populations.
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DOI：
10.7554/elife.63910
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发表时间：
2021-01-19
期刊：
影响因子：
7.7
通讯作者：
Desai MM
中科院分区：
文献类型：
--
作者：
Johnson MS;Gopalakrishnan S;Goyal J;Dillingham ME;Bakerlee CW;Humphrey PT;Jagdish T;Jerison ER;Kosheleva K;Lawrence KR;Min J;Moulana A;Phillips AM;Piper JC;Purkanti R;Rego-Costa A;McDonald MJ;Nguyen Ba AN;Desai MM
实验室实验进化提供了一个窗口，了解进化过程的细节。为了研究长期适应的后果，我们在三种环境中进化了205个酿酒酵母种群（124个单倍体和81个二倍体）约10，000代。我们测量了适应性随时间变化的动态，发现了适应性下降的可重复模式。测序显示，这种表型适应与突变的稳定积累、广泛的遗传平行性和历史偶然性相结合。与E.大肠杆菌，我们没有观察到长期共存或高度升高的突变率的群体。我们发现，在二倍体种群的进化涉及固定的杂合突变和频繁的杂合性丢失事件。总之，这些结果有助于区分进化动力学的各个方面，这些方面可能是许多系统适应的一般特征，而这些特征是特定于单个生物和环境条件的。
Laboratory experimental evolution provides a window into the details of the evolutionary process. To investigate the consequences of long-term adaptation, we evolved 205 Saccharomyces cerevisiae populations (124 haploid and 81 diploid) for ~10,000 generations in three environments. We measured the dynamics of fitness changes over time, finding repeatable patterns of declining adaptability. Sequencing revealed that this phenotypic adaptation is coupled with a steady accumulation of mutations, widespread genetic parallelism, and historical contingency. In contrast to long-term evolution in E. coli, we do not observe long-term coexistence or populations with highly elevated mutation rates. We find that evolution in diploid populations involves both fixation of heterozygous mutations and frequent loss-of-heterozygosity events. Together, these results help distinguish aspects of evolutionary dynamics that are likely to be general features of adaptation across many systems from those that are specific to individual organisms and environmental conditions.