Testing the repatriated for SARS-Cov2: Should laboratory-based quarantine replace traditional quarantine?
Testing the repatriated for SARS-Cov2: Should laboratory-based quarantine replace traditional quarantine?
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DOI：
10.1016/j.tmaid.2020.101624
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发表时间：
2020-03-01
影响因子：
12
通讯作者：
Brouqui, Philippe
中科院分区：
文献类型：
--
作者：
Lagier, Jean Christophe;Colson, Philippe;Brouqui, Philippe
背景资料：由新型冠状病毒（COVID 2019，SARS-CoV 2）引起的呼吸道疾病于2019年12月底在中国湖北武汉开始持续流行。法国政府决定遣返居住在武汉的337名法国公民，并将他们隔离在本国。我们决定WA他们所有的SARS-Cov 2两次，以减少人群和决策者之间的焦虑。方法：我们调查了存在的SARS-CoV-19无症状携带者通过测试所有遣返的患者在第一个24小时内，他们抵达法国，并在第5天。在没有鼻/口咽拭子的情况下，从合并的鼻和口咽拭子液体或痰液中提取病毒RNA。然后使用几种实时逆转录（RT）-PCR检测SARS-CoV-2 RNA进行检测。结果：我们测试了337名乘客在第0天和第5天。所有SARS-CoV 2检测均为阴性。通过优化采样过程，按顺序发送样本和减少生物分析的时间尺度，我们能够在5小时内WA样本（包括采样，运输和生物测试）。结论：优化我们的程序减少了焦虑，并保证了人口和决策者。
Background: An ongoing epidemic of respiratory diseases caused by a novel coronavirus (COVID 2019, SARS-CoV2) started in Wuhan, Hubei, in China at the end of December 2019. The French government decided to repatriate the 337 French nationals living in Wuhan and place them in quarantine in their home country. We decided to WA them all for SARS-Cov2 twice in order to reduce anxiety among the population and decision-makers.Methods: We investigated the presence of SARS-CoV-19 in asymptomatic carriers by testing all repatriated patients within the first 24 h of their arrival in France and at day 5. Viral RNA was extracted from pooled nasal and oropharyngeal swab fluids or sputum in the absence of nasal/oropharyngeal swabs. Detection of SARS-CoV-2 RNA was then carried out using several real-time reverse transcription (RT)-PCR assays.Results: We tested 337 passengers at day 0 and day 5. All the tests for SARS-CoV2 were negative. By optimising the sampling process, sending samples sequentially and reducing the time-scale for biological analysis, we were able to WA the samples within 5 h (including sampling, shipment and biological tests).Conclusion: Optimising our procedures reduces anxiety and reassures the population and decision makers.