Isometric contraction of microvascular pericytes from mouse brain parenchyma
Isometric contraction of microvascular pericytes from mouse brain parenchyma
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DOI：
10.1016/j.mvr.2006.08.004
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发表时间：
2007-01-01
影响因子：
3.1
通讯作者：
Ito, Yuko
中科院分区：
文献类型：
--
作者：
Oishi, Kazuhiko;Kamiyashiki, Tsutomu;Ito, Yuko
从小鼠脑实质微血管中分离培养周细胞。一个单一的周细胞克隆的三维培养在胶原蛋白凝胶中，通过增加拉伸应力，导致重建狭窄的线状纤维。当这些纤维的收缩性进行等距评估时，它们响应乙酰胆碱（ACh）（1）或去甲肾上腺素收缩;这伴随着细胞内钙浓度（[Ca 2 +]（i））的增加。被乙酰胆碱预收缩的纤维被罂粟碱完全松弛，罂粟碱是一种平滑肌松弛剂。此外，毒蕈碱型ACh受体拮抗剂阿托品抑制ACh诱导的[Ca ~（2+）]（i）反应。本研究首次定量测量了小鼠脑实质微血管周细胞培养产生的收缩。(c)2006年爱思唯尔公司All rights reserved.
Pericytes were isolated and cultured from mouse cerebroparenchymal microvessels. A single pericyte clone was three-dimensionally cultured in a collagen gel by adding tensile stress, resulting in the reconstruction of narrow stringy fibers. When the contractility of these fibers was evaluated isometrically, they contracted in response to acetylcholine (ACh)(1) or noradrenaline; this was accompanied by an increase in intracellular calcium concentration ([Ca2+](i)). The fibers that were pre-contracted by ACh were completely relaxed by papaverine, which is a smooth-muscle relaxant. Moreover, the muscarinic ACh receptor-antagonist atropine depressed the [Ca2+](i) response that was induced by ACh. This study demonstrates for the first time the quantitative measurement of the contractions produced by cultured microvascular pericytes from mouse brain parenchyma. (c) 2006 Elsevier Inc. All rights reserved.