Second-order projection from the posterior lateral line in the early zebrafish brain
Second-order projection from the posterior lateral line in the early zebrafish brain
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DOI：
10.1186/1749-8104-1-4
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发表时间：
2006-11-29
影响因子：
3.6
通讯作者：
Ghysen, Alain
中科院分区：
文献类型：
--
作者：
Fame, Ryann M.;Brajon, Carole;Ghysen, Alain
背景资料：鱼类侧线系统毛细胞收集的机械感觉信息由感觉神经元收集并发送到同侧后脑。然后，这些信息通过二级投射传递到其他大脑结构。在成年人中，第二阶投影的一部分延伸到对侧后脑，而另一部分连接到中脑结构，圆枕tocularis.Results：在本文中，我们研究了第二阶投影从后侧线系统在胚胎晚期/早期幼虫斑马鱼。在受精后4天，感觉神经元的突触场可以被准确地靶向，从而允许对二级神经元进行非常可重复的标记。我们表明，二阶预测是高度定型的，他们根据不同的菱形身份，他们几乎完全偏侧。我们还发现，投射不仅延伸到对侧后脑和枕叶，而且还延伸到许多其他脑中心，包括中脑中控制凝视和姿势的核团，以及推测的丘脑核团。我们认为，在早期大脑发育中观察到的广泛连接揭示了大多数脊椎动物共有的基本支架，其中不同的亚群后来在不同的脊椎动物群体中得到加强。大剧目的投影目标提供了一个很有前途的系统来研究这种差异投影能力的遗传编码。
Background: Mechanosensory information gathered by hair cells of the fish lateral-line system is collected by sensory neurons and sent to the ipsilateral hindbrain. The information is then conveyed to other brain structures through a second-order projection. In the adult, part of the second-order projection extends to the contralateral hindbrain, while another part connects to a midbrain structure, the torus semicircularis.Results: In this paper we examine the second-order projection from the posterior lateral-line system in late embryonic/early larval zebrafish. At four days after fertilization the synaptic field of the sensory neurons can be accurately targeted, allowing a very reproducible labeling of second-order neurons. We show that second-order projections are highly stereotyped, that they vary according to rhombomeric identity, and that they are almost completely lateralized. We also show that the projections extend not only to the contralateral hindbrain and torus semicircularis but to many other brain centers as well, including gaze- and posture-controlling nuclei in the midbrain, and presumptive thalamic nuclei.Conclusion: We propose that the extensive connectivity observed in early brain development reveals a basic scaffold common to most vertebrates, from which different subsets are later reinforced in various vertebrate groups. The large repertoire of projection targets provides a promising system to study the genetic encoding of this differential projection capacity.