Regulation of ovarian ornithine decarboxylase by human chorionic gonadotrophin.
Regulation of ovarian ornithine decarboxylase by human chorionic gonadotrophin.
复制标题
人绒毛膜促性腺激素对卵巢鸟氨酸脱羧酶的调节。
DOI：
10.1152/ajpcell.1987.253.5.c687
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Pegg,AE
中科院分区：
文献类型：
--
作者：
Sertich,GJ;Persson,L;Pegg,AE
用人绒毛膜促性腺激素（hCG）处理29日龄雌性Sprague-Dawley大鼠，使鸟氨酸脱羧酶活性迅速增加。使用特定放射免疫测定法的测量结果表明，增加的活性可以通过鸟氨酸脱羧酶蛋白量的平行变化来解释。蛋白质含量的增加是由合成速率的增加引起的，因为激素处理没有改变鸟氨酸脱羧酶的半衰期。通过与cDNA探针杂交测定鸟氨酸脱羧酶的mRNA含量，发现蛋白质量的增加与mRNA量的变化相关。这些结果表明，用hCG处理通过增加该酶的mRNA的产生或稳定性来诱导大鼠卵巢中的鸟氨酸脱羧酶。鸟氨酸脱羧酶的增加导致卵巢中腐胺的增加，但没有增加多胺、亚精胺和精胺的含量。这些研究结果表明，尽管其快速和大规模的诱导，鸟氨酸脱羧酶是不是限速步骤，确定这些多胺在该组织中的含量。他们还表明腐胺本身可能在卵巢中起重要作用。
Treatment of 29-day-old female Sprague-Dawley rats with human chorionic gonadotrophin (hCG) produced a large and rapid increase in the activity of ornithine decarboxylase. Measurements that use a specific radioimmunoassay showed that the increased activity could be accounted for by a parallel change in the amount of ornithine decarboxylase protein. The increased protein content was caused by an increased rate of synthesis, since the half-life of ornithine decarboxylase was not changed by the hormone treatment. The content of mRNA for ornithine decarboxylase was determined by hybridization with a cDNA probe, and it was found that the increased amount of protein was correlated with a change in the amount of mRNA. These results indicate that treatment with hCG induces ornithine decarboxylase in the rat ovary by increasing the production or the stability of the mRNA for this enzyme. The increased amount of ornithine decarboxylase led to an increase in putrescine in the ovary but did not increase the content of the polyamines spermidine and spermine. These findings show that, despite its rapid and large scale induction, ornithine decarboxylase is not the rate-limiting step that determines the content of these polyamines in this tissue. They also suggest that putrescine itself may play an important role in the ovary.