Transcriptomically Guided Mesendoderm Induction of Human Pluripotent Stem Cells Using a Systematically Defined Culture Scheme
Transcriptomically Guided Mesendoderm Induction of Human Pluripotent Stem Cells Using a Systematically Defined Culture Scheme
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DOI：
10.1016/j.stemcr.2019.11.001
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发表时间：
2019-12-10
影响因子：
5.9
通讯作者：
Stanford, William L.
中科院分区：
文献类型：
--
作者：
Carpenedo, Richard L.;Kwon, Sarah Y.;Stanford, William L.
人多能干细胞（hPSC）是组织工程、发育研究和疾病建模中的重要细胞来源。用于hPSC的快速谱系诱导的有效的、广泛适用的方案在干细胞生物学领域中引起了极大的兴趣。我们描述了一种简单、稳健的方法，用于在限定的条件下利用单细胞接种（SCS）和BMP 4和激活素A（BA）处理将hPSC分化为中内胚层。BA治疗很容易纳入现有的协议，软骨和内皮祖细胞分化，而微调BA条件有利于明确的内胚层承诺。在体外或体内长时间分化后，BA预处理导致以外胚层形成为代价的较高的中胚层和内胚层水平。这些数据表明，用BA处理的SCS是用于诱导中内胚层的有效方法，其可适用于中胚层和内胚层分化。
Human pluripotent stem cells (hPSCs) are an essential cell source in tissue engineering, studies of development, and disease modeling. Efficient, broadly amenable protocols for rapid lineage induction of hPSCs are of great interest in the stem cell biology field. We describe a simple, robust method for differentiation of hPSCs into mesendoderm in defined conditions utilizing single-cell seeding (SCS) and BMP4 and Activin A (BA) treatment. BA treatment was readily incorporated into existing protocols for chondrogenic and endothelial progenitor cell differentiation, while fine-tuning of BA conditions facilitated definitive endoderm commitment. After prolonged differentiation in vitro or in vivo, BA pretreatment resulted in higher mesoderm and endoderm levels at the expense of ectoderm formation. These data demonstrate that SCS with BA treatment is a powerful method for induction of mesendoderm that can be adapted for use in mesoderm and endoderm differentiation.