Quantitative Modulation of Polycomb Silencing Underlies Natural Variation in Vernalization
Quantitative Modulation of Polycomb Silencing Underlies Natural Variation in Vernalization
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DOI：
10.1126/science.1221881
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发表时间：
2012-08-03
期刊：
影响因子：
56.9
通讯作者：
Dean, Caroline
中科院分区：
文献类型：
--
作者：
Coustham, Vincent;Li, Peijin;Dean, Caroline
拟南芥品种已经适应了广泛气候的生长。开花和对齐春化反应与冬季长度的变化是这种适应的核心。春化作用涉及通过保守的Polycomb（PRC 2）机制使花阻遏物FLC的表观遗传沉默，该机制涉及组蛋白H3（H3 K27 me 3）上的Lys（27）的三甲基化。我们发现，响应冬季长度的变化映射到顺式多态性内FLC。一个罕见的组合，四个多态性周围的PHD-Polycomb复杂的成核区域定位确定需要更长的冷。染色质免疫沉淀实验表明，这些多态性的影响积累的H3 K27 me 3在拟南芥登录Lov-1，无论是在成核位点和基因体。通过顺式变异对染色质沉默的定量调节可能是导致进化变化的一般机制。
Arabidopsis thaliana accessions have adapted to growth in a wide range of climates. Variation in flowering and alignment of vernalization response with winter length are central to this adaptation. Vernalization involves the epigenetic silencing of the floral repressor FLC via a conserved Polycomb (PRC2) mechanism involving trimethylation of Lys(27) on histone H3 (H3K27me3). We found that variation for response to winter length maps to cis polymorphism within FLC. A rare combination of four polymorphisms localized around the nucleation region of a PHD-Polycomb complex determines a need for longer cold. Chromatin immunoprecipitation experiments indicate that these polymorphisms influence the accumulation of H3K27me3 in Arabidopsis accession Lov-1, both at the nucleation site and over the gene body. Quantitative modulation of chromatin silencing through cis variation may be a general mechanism contributing to evolutionary change.