A myosin I isoform in the nucleus
A myosin I isoform in the nucleus
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DOI：
10.1126/science.290.5490.337
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发表时间：
2000-10-13
期刊：
影响因子：
56.9
通讯作者：
de Lanerolle, P
中科院分区：
文献类型：
--
作者：
Pestic-Dragovich, L;Stojiljkovic, L;de Lanerolle, P
肌球蛋白I β的核异构体含有一个独特的16个氨基酸的氨基末端延伸已被确定。对16个氨基酸肽的亲和纯化抗体显示出核染色。共聚焦显微镜和电子显微镜显示核肌球蛋白I β与RNA聚合酶II共定位，对α -amanitin和放线菌素d敏感。该抗体共免疫沉淀RNA聚合酶II并阻断体外RNA合成。肌球蛋白I β的这一异构体似乎与RNA聚合酶II形成复合体，并可能影响转录。
A nuclear isoform of myosin I beta that contains a unique 16-amino acid aminoterminal extension has been identified. An affinity-purified antibody to the 16-amino acid peptide demonstrated nuclear staining. Confocal and electron microscopy revealed that nuclear myosin I beta colocalized with RNA polymerase II in an alpha-amanitin- and actinomycin D-sensitive manner. The antibody coimmunoprecipitated RNA polymerase II and blocked in vitro RNA synthesis. This isoform of myosin I beta appears to be in a complex with RNA polymerase II and may affect transcription.