Soil and plant specific effects on bacterial community composition in the rhizosphere
Soil and plant specific effects on bacterial community composition in the rhizosphere
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DOI：
10.1016/s0038-0717(01)00052-9
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发表时间：
2001-09-01
影响因子：
9.7
通讯作者：
Crowley, DE
中科院分区：
文献类型：
--
作者：
Marschner, P;Yang, CH;Crowley, DE
研究了植物根际真细菌群落结构与植物种类、土壤类型和根区位置的关系。三种植物（鹰嘴豆、油菜和苏丹草）生长在三种加利福尼亚土壤（砂质土壤、砂质壤土和粘土）的完整核心中，并提供含氮或不含氮的肥料溶液，如硝酸铵。7.5周后，收获植株，从根尖附着的土壤和侧根出芽部位的成熟根区提取DNA。采用16S rDNA PCR-DGGE检测真菌体群落结构，确定其相对丰度和物种多样性。土壤类型和施氮量对植物生长均有影响，但典型对应分析表明，施氮量对真细菌群落结构影响不显著。在沙质土和粘土中，成熟根带的真细菌物种多样性高于根尖，而在壤土中则低于根尖。蒙特卡罗排列试验表明，植物种类、根带和土壤类型及其相互作用对群落结构有显著影响。鹰嘴豆根际细菌群落主要受土壤类型的影响，而根区影响较小。与鹰嘴豆相比，油菜和苏丹草根际群落受根区影响大于土壤类型。在砂质土和壤土中，根区对真菌根际群落结构的影响大于植物种类，3种植物具有明显的群落特征。而在粘土中，根区对植物种类的影响较小，鹰嘴豆的根际群落与油菜和苏丹草的根际群落存在差异。因此，根际细菌群落组成受土壤类型、植物种类和根区位置的复杂相互作用影响。2001爱思唯尔科学有限公司版权所有。
Eubacterial community structures in the plant rhizosphere were examined with respect to plant species, soil type, and root zone location. Three plant species (chickpea, rape and Sudan grass) were grown in intact cores of three California soils (a sandy soil, a sandy loam, and a clay) and were provided with a complete fertilizer solution with or without nitrogen supplied as ammonium nitrate. After 7.5 weeks, the plants were harvested and DNA was extracted from soil adhering to the root tips and from mature root zones at the sites of lateral root emergence. Eubacterial community structures were examined by PCR-DGGE of 16S rDNA to determine the relative abundance and species diversity. While both soil type and nitrogen fertilization affected plant growth, canonical correspondence analyses showed that nitrogen had no significant effect on eubacterial community structures. Eubacterial species diversity was higher in the mature root zones than at the root tips in the sandy soil and the clay but not in the loamy sand. Monte Carlo permutation tests indicated that plant species, root zone and soil type as well as the interactions between these variables had significant effects on community structure. The bacterial rhizosphere community of chickpea was influenced primarily by soil type, whereas root zone was less important. In contrast to chickpea, the community in the rhizosphere of rape and Sudan grass was more affected by the root zone than the soil type. In the sandy soil and the loamy sand, the eubacterial rhizosphere community structure was more affected by the root zone than the plant species and the three plant species had distinct communities. In the clay however, the root zone was less important than the plant species and the rhizosphere communities of chickpea differed from those of rape and Sudan grass. It is concluded that the bacterial community composition in the rhizosphere is affected by a complex interaction between soil type, plant species and root zone location. (C) 2001 Elsevier Science Ltd. All rights reserved.