Aβ Upregulates and Colocalizes with LGI3 in Cultured Rat Astrocytes
Aβ Upregulates and Colocalizes with LGI3 in Cultured Rat Astrocytes
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DOI：
10.1007/s10571-006-9127-8
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发表时间：
2007-03
影响因子：
4
通讯作者：
N. Kimura;Y. Ishii;Shingo Suzaki;T. Negishi;S. Kyuwa;Y. Yoshikawa
中科院分区：
文献类型：
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作者：
N. Kimura;Y. Ishii;Shingo Suzaki;T. Negishi;S. Kyuwa;Y. Yoshikawa
1.富含亮氨酸的胶质瘤失活（LGI）基因家族编码富含亮氨酸的重复序列（LRR）蛋白，这些蛋白被认为特异性参与蛋白质-蛋白质和蛋白质-基质相互作用。由于淀粉样β肽（Aβ）先前已被证明可诱导神经细胞中另一种LRR编码基因的表达，因此我们评估了Aβ如何影响大鼠原代大脑皮质培养物和星形胶质细胞培养物中LGI基因的表达。RT-PCR和WesternBlotting分析均显示Aβ可显著诱导大鼠星形胶质细胞表达LGI 3. Western Blotting分析还显示胶质细胞酸性蛋白（GFAP）和载脂蛋白E（ApoE）均显著增加，与Aβ诱导的LGI 3上调一致。免疫细胞化学结果显示，LGI 3与Aβ共定位于质膜，也与星形胶质细胞内的Aβ共定位。这些结果表明，活化的LGI 3可能参与了星形胶质细胞对Aβ的反应。
1. The leucine-rich glioma inactivated (LGI) family of genes encodes a leucine-rich repeat (LRR) protein, proteins that are thought to be specifically involved in protein–protein and protein–matrix interactions. Since amyloid beta peptide (Aβ) has been previously shown to induce the expression of another LRR-encoding gene in neural cells, we assessed how Aβ affects LGI gene expression in rat primary cerebral cortical cultures and astrocyte cultures. Both RT-PCR and Western Blotting analyses revealed that Aβ robustly induced the expression of LGI3 in rat astrocyte cultures.2. Western Blotting analyses also showed that both glial fibrillary acidic protein (GFAP) and apolipoprotein E (ApoE) significantly increased coincidentally with the Aβ-induced upregulation of LGI3. Immunocytochemistry showed that LGI3 colocalized with Aβ at plasma membranes and also with internalized Aβ in astrocytes. These findings suggest that activated LGI3 may be involved in the astroglial response against Aβ.