SUPPRESSORS OF TEMPERATURE-SENSITIVE MUTATIONS IN A RIBOSOMAL-PROTEIN GENE, RPSL (S12), OF ESCHERICHIA-COLI-K12
SUPPRESSORS OF TEMPERATURE-SENSITIVE MUTATIONS IN A RIBOSOMAL-PROTEIN GENE, RPSL (S12), OF ESCHERICHIA-COLI-K12
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DOI：
10.1007/bf00330746
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发表时间：
1985-01-01
期刊：
影响因子：
--
通讯作者：
UCHIDA, H
中科院分区：
文献类型：
--
作者：
NASHIMOTO, H;MIURA, A;UCHIDA, H
Temperature-sensitive (ts) mutations were isolated within a ribosomal protein gene (rpsL) of E. coli K12. Mutations were mapped by complementation using various transducing phages and plasmids carrying the rpsL gene, having a normal or a defective promoter for the rpsL operon. One of these mutations, ts118, resulted in a mutant S12 protein which behaved different from the wild-type S12 on CM-cellulose column chromatography. Suppressors of these ts mutations were isolated and characterized; one was found to be a mutation of a non-ribosomal protein gene which was closely linked to the RNAase III gene on the E. coli chromosome. This suppressor, which was recessive to its wild-type allele, wa cloned into a transducing phae and mapped finely. A series of cold-sensitive mutations, affecting the assembly of ribosomes at 20.degree. C, was isolated within the purL to nadB region of the E. coli chromosome and 1 group, named rbaA, mapped at the same locus as the suppressor mutation, showing close linkage to the RNAase III gene.
