Combined effect of temperature and zinc on Caenorhabditis elegans wild type and daf-21 mutant strains.
Combined effect of temperature and zinc on Caenorhabditis elegans wild type and daf-21 mutant strains.
复制标题
DOI：
10.1016/j.jtherbio.2014.02.007
复制
发表时间：
2014-04
影响因子：
2.7
通讯作者：
Yunbiao Wang;Anastasia N Ezemaduka
中科院分区：
文献类型：
--
作者：
Yunbiao Wang;Anastasia N Ezemaduka
水生态系统中的重金属污染是一个影响深远的环境问题。重金属暴露的可能影响以及与其他环境应激因素（如温度）结合时对生物体的潜在危害在很大程度上尚未探索。进行秀丽隐杆线虫的水生毒性试验以估计不同锌浓度在不同温度（15 °C、20 °C、25 °C和30 °C）下的24 h半数致死浓度（LC 50）。我们还研究了在37 °C下暴露于6.1 mM锌的野生型（N2）和daf-21 null（JT 6130）成虫的时间过程热耐受性。Hsp 90蛋白表达水平的温度和锌毒性的联合作用进行了研究，通过Western印迹和ELISA。结果表明，C. eleganswild型线虫在较高温度下暴露于锌24小时后表现出严重的致死毒性。此外，Hsp 90的表达水平受到高度抑制，在成年蠕虫受到锌胁迫。这种在不同温度下的毒性测定提供了对温度和锌毒性的组合效应的生物体响应的深入了解。
Heavy metal pollution in aquatic ecosystems is a far reaching environmental problem. The possible influences of heavy metal exposure and the potential harm to organisms when combined with other environmental stressors such as temperature have been largely unexplored. An aquatic toxicity test of Caenorhabditis elegans was performed to estimate the 24 h median lethal concentration (LC50) of different zinc concentrations at different temperatures (15 °C, 20 °C, 25 °C, and 30 °C). We also examined the time course thermotolerance on wild type (N2) anddaf-21null (JT6130) adults exposed to 6.1 mM zinc at 37 °C. Hsp90 protein expression level in response to the combined effect of temperature and zinc toxicity was also investigated by both Western blots and ELISA. Our results show thatC. eleganswild type nematodes exhibit severe lethal toxicity after a 24 h exposure to zinc at higher temperatures. In addition, the expression level of Hsp90 was highly inhibited in adult worms subjected to zinc stress. This toxicity assay at different temperatures provides insight into organism response to combined effects of temperature and zinc toxicity.