A RETROTRANSPOSON-412 INSERTION WITHIN AN EXON OF THE DROSOPHILA-MELANOGASTER VERMILION GENE IS SPLICED FROM THE PRECURSOR RNA
A RETROTRANSPOSON-412 INSERTION WITHIN AN EXON OF THE DROSOPHILA-MELANOGASTER VERMILION GENE IS SPLICED FROM THE PRECURSOR RNA
复制标题
DOI：
10.1101/gad.4.4.559
复制
发表时间：
1990-04-01
影响因子：
10.5
通讯作者：
SEARLES, LL
中科院分区：
文献类型：
--
作者：
FRIDELL, RA;PRET, AM;SEARLES, LL
果蝇黑腹红唇(V)基因的三个等位基因被貂[su(S)]基因抑制子的隐性突变所抑制。以前的工作已经证实，这些等位基因在基因的5‘-非翻译区有相同的412反转录转座子插入。尽管在外显子中插入了转座子，但v突变体在su(S)+背景下积累了少量明显的野生型转录本，并且su(S)突变增加了v转录本的积累水平。在这里，我们通过S1核酸酶保护实验和对聚合酶链式反应产生的cDNA克隆的序列分析，鉴定了一个可抑制的v突变体在苏(S)+和苏(S)-背景下的转录本。我们发现，通过在412反转录转座子末端附近的供体和受体位置剪接，转座子序列从v突变转录本中被不精确地消除。将四个不同的5‘’供体部位交替剪接到单个3‘’受体部位。这一发现的含义被讨论与苏(S)+基因产物的可能功能有关。
Three alleles of the Drosophila melanogaster vermilion (v) gene are suppressed by recessive mutations at the suppressor of sable [su(s)], gene. Previous work has established that these alleles have identical insertions of the 412 retrotransposon in the 5''-untranslated region of the gene. Despite the transposon insertion in an exon, v mutants accumulate trace amounts of apparently wild-type-sized transcripts in a su(s)+ background, and the level of v transcript accumulation is increased by su(s) mutations. Here, we have characterized transcripts from a suppressible v mutant in both su(s)+ and su(s)- backgrounds by S1 nuclease protection experiments and sequence analysis of polymerase chain reaction (PCR) generated cDNA clones. We find that transposon sequences are imprecisely eliminated from v mutant transcripts by splicing at donor and acceptor sites located near the end of the 412 retrotransposon. Four different 5'' donor sites are alternatively spliced to a single 3'' acceptor site. The implications of this finding are discussed in relation to possible functions of the su(s)+ gene product.