FT-IR microscopic analysis of changing cellulose crystalline structure during wood cell wall formation
FT-IR microscopic analysis of changing cellulose crystalline structure during wood cell wall formation
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DOI：
10.1021/ma970768c
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发表时间：
1998-02-10
期刊：
影响因子：
5.5
通讯作者：
Kondo, T
中科院分区：
文献类型：
--
作者：
Kataoka, Y;Kondo, T
采用傅里叶变换红外光谱仪对针叶树管胞细胞壁形成各发育阶段纤维素晶体结构进行了研究。光谱表明，初生细胞壁中的纤维素富含亚稳的I-α晶型，有趣的是，其结晶度高于主要由稳定的I-β晶相组成的次生壁纤维素。这些结果表明，在结晶过程中，在细胞表面存在一种体内物理力，可能会对初生壁纤维素产生应力。初生壁纤维素的取向与生长细胞的膨大方向平行。因此，我们认为，细胞生长的压力之间产生的质膜和初级壁可能会延长刚刚生物合成的β-葡聚糖链，并导致它们结晶与较高的结晶度的亚稳I-α相，是在聚合物凝胶的拉伸过程中发生的结晶的情况。
A Fourier transform infrared (FT-IR) spectrometer equipped with a microscopic accessory was used to monitor the cellulose crystalline structure at each developmental stage of coniferous tracheid cell wall formation. The spectra showed that the cellulose in the primary cell wall was rich in the metastable I-alpha crystalline form and interestingly had a higher crystallinity than the secondary wall cellulose composed mainly of the stable I-beta crystalline phase. These results indicate the presence of an in vivo physical force at the cell surface which may stress the primary wall cellulose during the crystallization. Moreover, the primary wall cellulose was oriented parallel to the enlarging direction of growing cells. Thus, we consider that the cellular growing stress generated between the plasma membrane and the primary wall may elongate just-biosynthesized beta-glucan chains and cause them to crystallize with a higher crystallinity of the metastable I-alpha phase, as is the case for crystallization occurring during the drawing of polymer gels.