Characterisation of Saccharomyces cerevisiae ARO8 and ARO9 genes encoding aromatic aminotransferases I and II reveals a new aminotransferase subfamily
Characterisation of Saccharomyces cerevisiae ARO8 and ARO9 genes encoding aromatic aminotransferases I and II reveals a new aminotransferase subfamily
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DOI：
10.1007/s004380050644
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发表时间：
1998-01-01
期刊：
影响因子：
--
通讯作者：
Urrestarazu, A
中科院分区：
文献类型：
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作者：
Iraqui, I;Vissers, S;Urrestarazu, A
通过互补芳香族氨基转移酶I（aro 8）和II（aro 9）缺陷的aro 8 aro 9双突变菌株的苯丙氨酸/酪氨酸营养缺陷型分离酿酒酵母的ARO 8和ARO 9基因。基因测序，缺失突变体的构建和分析。研究ARO 8和ARO 9的表达。Aro 8 p和Aro 9 p的氨基酸序列推测前者为500个氨基酸残基的多肽，分子量为56168-Da，后者为513个氨基酸残基的多肽，分子量为58516-Da。它们分别对应于Yg 1202 p和Yhr 137 p，这两种假定的未知功能的蛋白质是通过酵母基因组的系统测序揭示的。我们发现，芳香族氨基转移酶I和II是同源蛋白质，氨基转移酶亚组I的成员，并且，与其他三种蛋白质一起，它们在亚组内构成了一个新的亚家族的酶专门用于芳香族氨基酸和α-氨基己二酸转氨。ARO 8的表达受氨基酸生物合成的一般控制。当芳香族氨基酸存在于生长培养基中时，ARO 9表达被诱导，并且在基本氨培养基上生长的aro 8突变体中也是如此。自主复制序列（ARS）元件位于ARO 8基因和YGL 201 c之间，YGL 201 c编码微小染色体维持家族的蛋白质。
The ARO8 and ARO9 genes of Saccharomyces cerevisiae were isolated by complementation of the phenylalanine/tyrosine auxotrophy of an aro8 aro9 double-mutant strain that is defective in aromatic aminotransferases I (aro8) and II (aro9). The genes were sequenced, and deletion mutants were constructed and analysed. The expression of ARO8 and ARO9 was studied. The deduced amino acid sequences of Aro8p and Aro9p suggest that the former is a 500-residue, 56168-Da polypeptide and the latter a 513-residue, 58516-Da polypeptide. They correspond, respectively, to Yg1202p and Yhr137p, two putative proteins of unknown function revealed by systematic sequencing of the yeast genome. We show that aromatic aminotransferases I and II are homologous proteins, members of aminotransferase subgroup I, and, together with three other proteins, they constitute within the subgroup a new subfamily of enzymes specialised for aromatic amino acid and alpha-aminoadipate transamination. ARO8 expression is subject to the general control of amino acid biosynthesis. ARO9 expression is induced when aromatic amino acids are present in the growth medium and also in aro8 mutants grown on minimal ammonia medium. An autonomously replicating sequence (ARS) element is located between the ARO8 gene and YGL201c which encodes a protein of the minichromosome maintenance family.