Generation of a bioengineered tooth by using a three-dimensional cell manipulation method (organ germ method).
Generation of a bioengineered tooth by using a three-dimensional cell manipulation method (organ germ method).
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DOI：
10.1007/978-1-61779-860-3_14
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发表时间：
2012-01-01
期刊：
影响因子：
--
通讯作者：
Tsuji, Takashi
中科院分区：
文献类型：
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作者：
Oshima, Masamitsu;Ogawa, Miho;Tsuji, Takashi
组织内细胞的排列在器官发生（包括牙齿发育）中起着至关重要的作用。众所周知，由三维组织结构诱导的器官形态发生和生理功能受各种信号分子（包括细胞因子、细胞外基质蛋白和粘附分子）的适当时空结构的调节。开发三维细胞操作技术以创建生物工程化器官胚，称为器官胚方法，使得能够在体内生成结构正确且功能齐全的生物工程化牙齿。该方法有望成为分析器官发生过程中基因和蛋白质功能的一种有价值的技术。在这里，我们描述了使用器官胚方法的牙胚重建方案和体外和体内牙齿发育分析方法。
The arrangement of cells within a tissue plays an essential role in organogenesis, including tooth development. Organ morphogenesis and physiological functions induced by three-dimensional tissue organization are well known to be regulated by the proper spatiotemporal organization of various signaling molecules, including cytokines, extracellular matrix proteins, and adhesion molecules. Development of a three-dimensional cell manipulation technology to create a bioengineered organ germ, designated as the organ germ method, enabled the generation of a structurally correct and fully functional bioengineered tooth in vivo. This method is expected to be utilized as a valuable technique for analyzing gene and protein functions during organogenesis. Here, we describe protocols for tooth germ reconstitution using the organ germ method and methods for analyzing tooth development in vitro and in vivo.