Gold nanoshell bioconjugates for molecular imaging in living cells
Gold nanoshell bioconjugates for molecular imaging in living cells
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DOI：
10.1364/ol.30.001012
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发表时间：
2005-05-01
期刊：
影响因子：
3.6
通讯作者：
Drezek, R
中科院分区：
文献类型：
--
作者：
Loo, C;Hirsch, L;Drezek, R
基于散射的光学成像技术的进步为癌症的无创即时检测、诊断和监测提供了新的方法。新兴的光子学技术提供了一种具有成本效益的手段，以真实的时间对体内组织进行高分辨率成像。推进这些成像策略的临床潜力需要开发针对疾病特定分子特征的光学造影剂。我们描述了使用一类新的造影剂的基础上纳米壳生物共轭分子成像活细胞。与传统成像探针相比，纳米壳具有显著的优势，包括连续和宽波长可调谐性、更大的散射和吸收系数、更高的化学稳定性和更好的生物相容性。我们表明，纳米壳生物缀合物可用于有效地靶向和成像人表皮生长因子受体2（HER2），一种临床相关的生物标志物，在活的人乳腺癌细胞。(c)2005年，美国光学学会。
Advances in scattering-based optical imaging technologies offer a new approach to noninvasive point-of-care detection, diagnosis, and monitoring of cancer. Emerging photonics technologies provide a cost-effective means to image tissue in vivo with high resolution in real time. Advancing the clinical potential of these imaging strategies requires the development of optical contrast agents targeted to specific molecular signatures of disease. We describe the use of a novel class of contrast agents based on nanoshell bioconjugates for molecular imaging in living cells. Nanoshells offer significant advantages over conventional imaging probes including continuous and broad wavelength tunability, far greater scattering and absorption coefficients, increased chemical stability, and improved biocompatibility. We show that nanoshell bioconjugates can be used to effectively target and image human epidermal growth factor receptor 2 (HER2), a clinically relevant biomarker, in live human breast carcinoma cells. (c) 2005 Optical Society of America.