Levels of Interferon-Gamma and Transcription Factor T-Bet in Progressive Periodontal Lesions in Patients With Chronic Periodontitis
Levels of Interferon-Gamma and Transcription Factor T-Bet in Progressive Periodontal Lesions in Patients With Chronic Periodontitis
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DOI：
10.1902/jop.2009.080287
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发表时间：
2009-02-01
影响因子：
4.3
通讯作者：
Gamonal, Jorge
中科院分区：
文献类型：
--
作者：
Dutzan, Nicolas;Vernal, Rolando;Gamonal, Jorge
背景：牙周炎是一种以组织支持破坏为特征的偶发性感染。在初级CD 4（+）辅助性T细胞1（Th 1）应答的产生过程中，干扰素-γ（IFN-γ）通过增加T-bet的转录选择性诱导Th 1分化而充当正调节剂。这项工作的目的是确定IFN-γ水平的样本中的龈沟液（GCF），并确定IFN-γ和转录因子T-bet的表达在牙龈组织中的患者经历的进展慢性牙周炎。方法：106例中度或晚期慢性牙周炎被选中。牙周炎的特征是至少有6个部位的探测深度>= 5 mm，临床附着丧失>= 3 mm，放射学骨丧失。通过耐受性方法确定牙周炎进展。使用试纸条收集GCF，并进行酶联免疫吸附测定以测定IFN-γ的总量。从患者的牙龈活检中获得实时逆转录聚合酶链反应，以确定IFN-γ和T-bet表达。使用统计软件进行统计分析。数据表示为受试者平均值SD。结果：细胞因子IFN-γ在活性部位的总量和浓度显著高于非活性部位（分别为99.90和68.90 pg; P = 0.03; 106.62 pg/mg和75.64 pg/mg，P = 0.04）。活性位点的IFN-γ δ循环阈值（Ct）显著低于非活性位点（P= 0.04），而转录因子T-bet在活性位点的表达是非活性位点的1.42倍。GCF样品中细胞因子IFN-γ和转录因子T-IFN-γ的总量和浓度在GCF样品中是显著的。慢性牙周炎患者进行性牙周病变中bet表达增加。J Periodontol 2009;80：290-296.
Background: Periodontitis is an infection with an episodic pattern of tissue-support destruction. During the generation of a primary CD4(+) T helper 1 (Th1) response, interferon-gamma (IFN-gamma) acts as a positive regulator by selectively inducing Th1 differentiation through increased transcription of T-bet. The aims of this work were to determine IFN-gamma levels in samples of gingival crevicular fluid (GCF) and to determine IFN-gamma and transcription factor T-bet expression in gingival tissue from patients undergoing the progression of chronic periodontitis.Methods: One hundred six patients with moderate or advanced chronic periodontitis were selected. Periodontitis was characterized by at least six sites with probing depth >= 5 mm, clinical attachment loss >= 3 mm, and radiographic bone loss. Periodontitis progression was determined by the tolerance method. GCF was collected using a paper strip, and enzyme-linked immunosorbent assay was performed to determine the total amount of IFN-gamma. Gingival biopsies were obtained from patients for real-time reverse transcription-polymerase chain reaction to determine IFN-gamma and T-bet expression. Statistical analysis was performed using statistical software. Data were expressed as subject means SD. The chi(2) and Student t tests were used.Results: The total amount and concentration of cytokine IFN-gamma were significantly higher in active sites than in inactive sites (99.90 versus 68.90 pg; P = 0.03; 106.62 pg/mg versus 75.64 pg/mg, P = 0.04, respectively). Active sites showed a significantly lower Delta cycle threshold (Ct) of IFN-gamma than inactive sites (P= 0.04), whereas the expression of transcription factor T-bet was increased 1.42-fold in active sites compared to inactive sites.Conclusion: The total amount and concentration of cytokine IFN-gamma in GCF samples and transcription factor T-bet expression were increased in progressive periodontal lesions in patients with chronic periodontitis. J Periodontol 2009;80:290-296.