Suppressor of gamma response 1 (SOG1) encodes a putative transcription factor governing multiple responses to DNA damage
Suppressor of gamma response 1 (SOG1) encodes a putative transcription factor governing multiple responses to DNA damage
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DOI：
10.1073/pnas.0810304106
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发表时间：
2009-08-04
影响因子：
11.1
通讯作者：
Britt, Anne B.
中科院分区：
文献类型：
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作者：
Yoshiyama, Kaoru;Conklin, Phillip A.;Britt, Anne B.
拟南芥sog1 - 1（γ反应抑制因子）突变体最初是作为修复核酸内切酶XPF缺陷的种子辐射敏感表型的第二位点抑制因子被分离出来的。在此，我们报道SOG1编码一种假定的转录因子。该基因是NAC结构域[矮牵牛NAM（无顶端分生组织）以及拟南芥ATAF1、2和CUC2]家族（陆生植物特有的一个蛋白质家族）的成员。在电离辐射处理后的一小时内，拟南芥中通常有数百个基因上调；这些基因的诱导需要损伤反应蛋白激酶ATM，但不需要相关的激酶ATR。在此，我们发现这种转录上调也需要SOG1。相反，在xpf突变体种子中观察到的依赖于SOG1的检查点反应需要ATR，但不需要ATM。因此，sog1 - 1突变体的表型模拟了拟南芥中atr和atm突变体表型的某些方面，这表明SOG1参与了由这两种传感器激酶调控的途径。我们提出，在植物中，与基因组应激相关的信号通过一个单一的核心转录因子SOG1进行处理。
The Arabidopsis sog1-1 (suppressor of gamma response) mutant was originally isolated as a second-site suppressor of the radiosensitive phenotype of seeds defective in the repair endonuclease XPF. Here, we report that SOG1 encodes a putative transcription factor. This gene is a member of the NAC domain [petunia NAM (no apical meristem) and Arabidopsis ATAF1,2 and CUC2] family (a family of proteins unique to land plants). Hundreds of genes are normally up-regulated in Arabidopsis within an hour of treatment with ionizing radiation; the induction of these genes requires the damage response protein kinase ATM, but not the related kinase ATR. Here, we find that SOG1 is also required for this transcriptional up-regulation. In contrast, the SOG1-dependent checkpoint response observed in xpf mutant seeds requires ATR, but does not require ATM. Thus, phenotype of the sog1-1 mutant mimics aspects of the phenotypes of both atr and atm mutants in Arabidopsis, suggesting that SOG1 participates in pathways governed by both of these sensor kinases. We propose that, in plants, signals related to genomic stress are processed through a single, central transcription factor, SOG1.