Determination of lupenone and β-sitosterol in Rhizoma Musae by UPLC with DAD and ELSD.
Determination of lupenone and β-sitosterol in Rhizoma Musae by UPLC with DAD and ELSD.
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DOI：
10.1093/chromsci/bmv079
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发表时间：
2015-11
影响因子：
1.3
通讯作者：
Feng Xu;Hongmei Wu;Yuanmin Wang;Ye Yang;Yuan Gao;Xiangpei Wang
中科院分区：
文献类型：
--
作者：
Feng Xu;Hongmei Wu;Yuanmin Wang;Ye Yang;Yuan Gao;Xiangpei Wang
芭蕉根在中国的苗族医学实践中已经使用了几个世纪，通常用于治疗糖尿病和擦伤。采用反相超高效液相色谱法（RP-UPLC）分离香蕉中的羽扇豆酮和β-谷甾醇，以甲醇和0.1%乙酸水溶液为流动相，二极管阵列检测器（DAD）和蒸发光散射检测器（ELSD）同时检测流动相为移动的，流速为0.1 mL/min，柱温为50°C，紫外检测波长为206 nm。注射体积为1.0 µL。ELSD的参数设定为45°C的探头温度，氮气雾化器调整为1.5 L/min。对RP-UPLC方法的准确度、精密度、检测限和定量限进行了验证。结果表明，DAD法和ELSD法均适用于芭蕉根中羽扇豆酮和β-谷甾醇的含量测定，且DAD法的灵敏度优于ELSD法。
Rhizoma Musae has been used for centuries in Miao medicine practice in China, and it usually uses for treating diabetes and bruises. In this study, lupenone and β-sitosterol in Rhizoma Musae were separated by reversed-phase ultra-performance liquid chromatography (RP-UPLC) and simultaneously detected by a diode array detector (DAD) and an evaporative light scattering detector (ELSD) using methanol and 0.1% aqueous acetic acid (100 : 4, v/v) as a mobile phase in 20 min. The flow rate of 0.1 mL/min was set with isocratic, the temperature of column compartment maintained at 50°C and ultraviolet detection set at 206 nm wavelength. The injection volume was 1.0 µL. The parameter for the ELSD was set to a probe temperature of 45°C, and the nebulizer for nitrogen gas was adjusted to 1.5 L/min. The RP-UPLC method was validated for accuracy, precision, limit of detection and limit of quantification. It applied to the quantification of the active chemical constituents of Rhizoma Musae, and results indicated that both DAD and ELSD were suitable for the determination of lupenone and β-sitosterol, and the DAD has a better sensitivity than the ELSD.