Distribution of gap junction protein connexin 37 in smooth muscle cells of the rat trachea and pulmonary artery.
Distribution of gap junction protein connexin 37 in smooth muscle cells of the rat trachea and pulmonary artery.
复制标题
间隙连接蛋白connexin 37在大鼠气管和肺动脉平滑肌细胞中的分布。
DOI：
10.1679/aohc.62.27
复制
发表时间：
1999
影响因子：
--
通讯作者：
Y. Shibata
中科院分区：
文献类型：
--
作者：
K. Nakamura;T. Inai;K. Nakamura;Y. Shibata
连接蛋白37是差距连接蛋白家族之一，已通过北方印迹在多种器官和组织中检测到，并且发现在肺中特别丰富。然而，关于表达连接蛋白37的细胞的确切类型的详细信息先前尚未发表。因此，我们制备了位点特异性连接蛋白37抗体，并以化学方法检测了连接蛋白37的分布。在肺动脉和主动脉的内皮细胞、图尼卡中膜以及气管和细支气管的平滑肌层中检测到连接蛋白37。在气管平滑肌层中，连接蛋白37与结蛋白阳性区域重叠，但与波形蛋白和血管性血友病因子阳性区域明显分离。这些结果表明，连接蛋白37在气管中的平滑肌细胞中表达，但不在成纤维细胞或内皮细胞中表达。连接蛋白37与连接蛋白43部分共存于气管平滑肌细胞中，并在出生后发育过程中表达逐渐增加。据我们所知，这是第一份关于连接蛋白37在平滑肌细胞中表达的报告。
Connexin 37, one of the gap junction protein families, has been detected by Northern blotting in various organs and tissues, and found to be especially abundant in the lung. However, detailed information on the precise types of cells which express connexin 37 has not been previously published. We therefore prepared site-specific connexin 37 antibodies and examined the distribution of connexin 37 immunohistochemically. Connexin 37 was detected in endothelial cells, in the tunica media of both the pulmonary artery and the aorta, and in the smooth muscle layer of the trachea and bronchioles. In the tracheal smooth muscle layer, connexin 37 overlapped with desmin-positive areas, but was clearly segregated from vimentin- and von Willebrand factor-positive areas. These results suggest that connexin 37 is expressed in smooth muscle cells in the trachea, but not in fibroblastic cells or endothelial cells. Connexin 37 was partially colocalized with connexin 43 in tracheal smooth muscle cells, and showed a gradual increase in expression during postnatal development. To our knowledge, this is the first report to be published regarding the expression of connexin 37 in smooth muscle cells.
间隙连接通道的分子生物学和遗传学。
DOI：
10.1016/s1043-4682(10)80003-0
发表时间：
1992
期刊：
Seminars in cell biology
影响因子：
--
作者：
Kumar,NM;Gilula,NB
通讯作者：
Gilula,NB