USE OF CONGO RED POLYSACCHARIDE INTERACTIONS IN ENUMERATION AND CHARACTERIZATION OF CELLULOLYTIC BACTERIA FROM THE BOVINE RUMEN
USE OF CONGO RED POLYSACCHARIDE INTERACTIONS IN ENUMERATION AND CHARACTERIZATION OF CELLULOLYTIC BACTERIA FROM THE BOVINE RUMEN
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DOI：
10.1128/aem.43.4.777-780.1982
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发表时间：
1982-01-01
影响因子：
4.4
通讯作者：
WOOD, PJ
中科院分区：
文献类型：
--
作者：
TEATHER, RM;WOOD, PJ
直接染料刚果红与完整的- β的相互作用。- d -葡聚糖为快速、灵敏地检测含有- β的菌株提供了基础。- (1 .fwdarw。4), (1)；3) -D-glucanohydrolase度量。- (1 .fwdarw。4)- d -葡聚糖水解酶和。- (1 .fwdarw。3) -D-glucanohydrolase活动。纤维素分解活性与- β密切相关。- (1 .fwdarw。4)- d -葡聚糖水解酶和。- (1 .fwdarw。4), (1)；3)牛瘤胃分离物- d -葡聚糖水解酶活性。这些分离物中的许多也含有。β。- (1 .fwdarw。3) -D-glucanohydrolase活动;这一特征可能具有分类学意义。
The interaction of the direct dye Congo red with intact .beta.-D-glucans provides the basis for a rapid and sensitive assay system for bacterial strains possessing .beta.-(1 .fwdarw. 4), (1 .fwdarw. 3)-D-glucanohydrolase, .beta.-(1 .fwdarw. 4)-D-glucanohydrolase and .beta.-(1 .fwdarw. 3)-D-glucanohydrolase activities. A close correspondence was observed between cellulolytic activity and .beta.-(1 .fwdarw. 4)-D-glucanohydrolase and .beta.-(1 .fwdarw. 4), (1 .fwdarw. 3)-D-glucanohydrolase activiries in isolates from the bovine rumen. Many of these isolates also possessed .beta.-(1 .fwdarw. 3)-D-glucanohydrolase activity; this characteristic may have taxonomic significance.