Bioavailability assessment of zinc enriched lactobacillus biomass in a human colon carcinoma cell line (Caco-2)
Bioavailability assessment of zinc enriched lactobacillus biomass in a human colon carcinoma cell line (Caco-2)
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DOI：
10.1016/j.foodchem.2019.125583
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发表时间：
2020-03-20
期刊：
影响因子：
8.8
通讯作者：
Arora, Sumit
中科院分区：
文献类型：
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作者：
Lule, Vaibhao Kisanrao;Tomar, Sudhir Kumar;Arora, Sumit
本研究利用具有在其生物质中积累显著量的锌（Zn）的潜力和以高度生物可利用的形式递送相同矿物质的能力的乳酸杆菌菌株。研究了人源发酵乳杆菌SR 4和鼠李糖乳杆菌GG（LGG）在含锌盐的培养基中生长对锌的富集能力。此外，通过超声处理制备富含Zn的细胞裂解物作为有机Zn源。通过FT-IR光谱确定了细菌锌结合的各种官能团，并通过SEM和能量色散X射线光谱（EDX）证实了生物螯合细胞裂解物复合物中的元素锌。实验数据表明，SR 4螯合的锌的生物利用度显著高于LGG螯合的锌的生物利用度（P < 0.05），57%和48%，相比之下，所测试的市售无机（ZnSO（4））和甚至有机（葡萄糖酸锌）形式分别为15.6%和21.7%。
The present study utilizes lactobacilli strains having the potential to accumulate a significant amount of Zinc (Zn) in their biomass and ability to deliver the same mineral in a highly bioavailable form. A human origin Lactobacillus fermentum SR4 and Lactobacillus rhamnosus GG (LGG) were studied for their ability to accumulate Zn by growing them in the medium containing Zn salt. Further, Zn enriched cell lysates were prepared by Ultrasonication, as an organic Zn source. Various functional groups involved in bacterial Zn binding were identified by FT-IR spectroscopy and elemental Zn in bio-chelated cell lysate complex was confirmed by SEM and Energy Dispersive X-ray Spectrometry (EDX). Experimental data demonstrated a significantly higher (P < 0.05) bioavailability of Zn chelated by SR4 followed by LGG i.e., 57% and 48%, as compared to the commercially available inorganic (ZnSo(4)) and even organic (Zinc gluconate) forms tested which has 15.6% and 21.7% respectively.