Chlorophyll fluorescence as a selection tool for cold tolerance of photosynthesis in maize (Zea mays L.)
Chlorophyll fluorescence as a selection tool for cold tolerance of photosynthesis in maize (Zea mays L.)
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DOI：
10.1093/jexbot/50.338.1533
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发表时间：
1999-09-01
影响因子：
6.9
通讯作者：
Stamp, P
中科院分区：
文献类型：
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作者：
Fracheboud, Y;Haldimann, P;Stamp, P
利用叶绿素a荧光猝灭分析作为提高玉米抗寒性的选择工具，研究了6个在低温条件下产生光合器官能力差异较大的基因型玉米的可能性。在逐渐冷却后，电子传递量子产率（Phi(PSII)）的测量表明，在次优温度（15℃）下发育的耐基因型叶片比敏感基因型叶片保持更高的电子传递率。这种差异主要是由于耐受性植物通过开放的光系统II反应中心（F'F- v '(m)）保持更高的激发能捕获效率的能力。在最适温度下发育的叶片没有基因型差异，表明该性状不是组成性表达，而是依赖于适应机制。此外，在15℃和25℃的光照增加下，基因型差异没有表达，这表明该性状也是低温特异性的，并不仅仅是对增加过量光能的响应。将该方法应用于在次优温度下生长的F-3近交系间杂交后代，其光合能力显著提高（最高可达31%），表明该方法是通过育种提高玉米耐寒性的有效选择工具。
The possibility of using quenching analysis of chlorophyll a fluorescence as a selection tool for improving the cold tolerance of maize was investigated in six genotypes differing greatly in the ability to develop a competent photosynthetic apparatus at low temperature. Upon gradual cooling, measurements of the quantum yield of electron transport (Phi(PSII)) indicated that leaves of tolerant genotypes, that developed at suboptimal temperature (15 degrees C), maintained higher rates of electron transport than leaves of sensitive genotypes. This difference was largely due to the ability of the tolerant plants to keep higher efficiency of excitation energy capture by open photosystem II reaction centres (F'F-V'(m)). The absence of genotypic differences in leaves that developed at optimal temperature indicates that the trait is not expressed constitutively, but relies on adaptation mechanisms, Furthermore, the genotypic difference was not expressed under increasing illumination at 15 degrees C and 25 degrees C suggesting that the trait is also low-temperature-specific and is not expressed solely in response to increasing excess light energy. Applying the method to flint and dent breeding population led to a substantial increase (up to 31%) in the photosynthetic capacity of hybrids between selected F-3 inbreeding families grown at suboptimal temperature, demonstrating that the method is an efficient selection tool for improving the cold tolerance of maize through breeding.