Sensitive analysis of aconitine, hypaconitine, mesaconitine and jesaconitine in human body fluids and Aconitum tubers by LC/ESI-TOF-MS
Sensitive analysis of aconitine, hypaconitine, mesaconitine and jesaconitine in human body fluids and Aconitum tubers by LC/ESI-TOF-MS
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DOI：
10.1002/jms.1038
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发表时间：
2006-06-01
影响因子：
2.3
通讯作者：
Ishii, Akira
中科院分区：
文献类型：
--
作者：
Kaneko, Rina;Hattori, Satoshi;Ishii, Akira
毛茛科（Ranunculaceae）毛茛属植物广泛分布于北方亚洲和北美洲。它们的根在中国和日本被广泛用于草药。有许多关于这种植物的意外、自杀和杀人中毒的报道;其中一些是致命的，因为这种植物的毒性非常高。因此，重要的是以高灵敏度检测和定量体液中的生物碱。建立了一种简便、灵敏的LC/ESI-飞行时间-质谱（TOF-MS）同时测定乌头碱、次乌头碱、耶乌头碱和中乌头碱的方法。这四种化合物和甲基甘草次酸（内标）在人血浆样品中进行了纯化，通过固相萃取。4种提取物在质量色谱图中完全分离，标准曲线在10-300 ng/ml范围内呈良好的线性关系，检出限为0.2-0.5 ng/ml。使用我们的方法，我们还确定了这些化合物在块茎样品中的量。本方法适用于临床和法医毒理学。版权所有（c）2006约翰威利父子有限公司。
The Aconitum species (Ranunculaceae) are widely distributed in northern Asia and North America. Their roots are popularly used in herbal medicines in China and Japan. Many cases of accidental, suicidal and homicidal intoxication with this plant have been reported; some of these were fatal because the toxicity of Aconitum is very high. It is thus important to detect and quantify Aconitum alkaloids in body fluids, with high sensitivity. We have developed a simple and sensitive method for measuring four kinds of Aconitum alkaloids (aconitine, hypaconitine, jesaconitine and mesaconitine) by LC/electrospray (ESI)-time-of-flight (TOF)-MS. For all of them, only molecular ions were observed at an orifice voltage of 75 V; at 135 V, base peaks corresponding to [M - 60 + H](+) ions were observed. These four compounds and methyllycaconitine (internal standard) in human plasma samples were purified by solid-phase extraction. The four extracted compounds were completely separated in mass chromatograms; the calibration curves showed good linearity in the range 10-300 ng/ml, and the detection limits were estimated to be 0.2-0.5 ng/ml. Using our method, we also determined the amounts of these compounds in tuber samples. The present method is applicable in clinical and forensic toxicology. Copyright (c) 2006 John Wiley & Sons, Ltd.