Region-specific neural induction of an engrailed protein by anterior notochord in Xenopus.
Region-specific neural induction of an engrailed protein by anterior notochord in Xenopus.
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非洲爪蟾前脊索对嵌入蛋白的区域特异性神经诱导。
DOI：
10.1126/science.1978411
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Harland,RM
中科院分区：
文献类型：
--
作者：
Hemmati-Brivanlou,A;Stewart,RM;Harland,RM
前部特异性神经诱导可以通过识别含Xenopushomeobox蛋白En-2的抗体来测定。En-2抗原是一个很好的早期标记，因为它是作为一个离散的带在神经胚的前神经板。通过结合背侧中胚层和感受态外胚层进行区域诱导。前脊索从早期神经胚诱导En-2频繁，而后脊索诱导En-2频率较低。假定体节中胚层和假定头部中胚层，虽然他们诱导神经组织，不是强诱导剂的En-2。因此，前脊索可能是主要的中胚层组织负责前神经板的图案。
Anterior-specific neural induction can be assayed by means of an antibody that recognizes theXenopushomeobox-containing protein En-2. The En-2 antigen is an excellent early marker, since it is present as a discrete band in the anterior neural plate of neurula embryos. Regional induction was assayed by combining dorsal mesoderm with competent ectoderm. Anterior notochord from the early neurula induced En-2 frequently, while posterior notochord induced En-2 less frequently. Presumptive somitic mesoderm and presumptive head mesoderm, though they induced neural tissue, were not strong inducers of En-2. Thus, anterior notochord may be the primary mesodermal tissue responsible for the patterning of the anterior neural plate.