Esterase. XXIII. Electron microscopical demonstration of non-specific esterases in the jejunum of the mouse (Mus musc.) with two quinoline derivatives.
Esterase. XXIII. Electron microscopical demonstration of non-specific esterases in the jejunum of the mouse (Mus musc.) with two quinoline derivatives.
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DOI：
10.1007/bf00492787
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发表时间：
1976-11-19
期刊：
影响因子：
--
通讯作者：
Deimling, O V
中科院分区：
文献类型：
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作者：
Bocking, A;Grossarth, C;Deimling, O V
以8-乙酰喹啉（Q-O-2）和8-乙酰巯基喹啉（Q-S-2）为底物，研究了非特异性酯酶在小鼠空肠各绒毛细胞内的分布。选择性更强的Q-S-2染色在所有肠细胞中表现出均匀的反应，主要位于内质网和核膜。这可能是一种酶很难从膜上分离出来的问题。对Q-O-2进行非选择性染色，发现了几种酯酶Es-2，其中有Es-9。主要反应发生在柱状细胞和杯状细胞中，在所有细胞膜和线粒体基质中，此外在内质网和高尔基体的池和囊泡中，以及在前者的脂滴中，最后在后者的粘液滴中。细胞外反应发生在细胞间隙和毛刷边缘的微绒毛之间。
The intracellular distribution of non specific esterases in various villous cells of mouse jejunum was investigated using two substrates, 8-acetoxiquinoline (Q-O-2) and 8-acetyl mercaptoquinoline (Q-S-2) respectively. With the more selectively staining Q-S-2 a uniform reaction was demonstrated in all enterocytes which was mainly located in the endoplasmic reticulum and the nuclear envelope. Possibly it is a matter of a single enzyme being hardly detachable from the membranes. With the non selectively staining Q-O-2 several esterases were demonstrated Es-2, adn Es-9 among them. The main reaction was found in columnar and Goblet cells, in both at all cellular membranes and in the mitochondrial matrix, additionally in cisterns and vesicles of the endoplasmic reticulum and of the Golgi apparatus and at lipid droplets of the former, finally at mucous droplets of the latter. An extracellular reaction was found in the intercellular cleft and between the microvilli of the brush border.