Major Anthocyanin Pigments in the Ficus padana Fruits: HPLC-DAD-ESI-MS Identification and Antioxidant Activity

Major Anthocyanin Pigments in the Ficus padana Fruits: HPLC-DAD-ESI-MS Identification and Antioxidant Activity
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巴达纳榕果实中的主要花青素色素:HPLC-DAD-ESI-MS 鉴定及其抗氧化活性

DOI:
10.22146/ijc.21242
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发表时间:
2014
期刊:
影响因子:
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通讯作者:
A. Santoni
A. Santoni
中科院分区:
--
文献类型:
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作者:
Daimon Syukri;D. Darwis;A. Santoni

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采用高效液相色谱-二极管阵列检测器(HPLC-DAD-ESI-MS)对莲花榕花色苷进行提取和鉴定。通过比较它们的质谱数据和已发表的数据来鉴定单个花青素。第一个化合物(峰1)被鉴定为天竺葵素3-(6”-对香豆基葡糖苷)-5-(4“”-丙二酰基葡糖苷),第二个化合物被鉴定为天竺葵素3-(6“”-丙二酰基葡糖苷)。第二种是主要成分,占花青苷总含量的91%。巴达那提取物采用2,2-二苯基-1-苦基肼(DPPH)法测定其抗氧化活性,结果表明该提取物具有较强的抗氧化能力。
The anthocyanins in Ficus padana were extracted and identified by using high-performance liquid chromatography/diode array detection and electrospray ionization/mass spectrometry (HPLC-DAD-ESI-MS). The individual anthocyanins were identified by comparing between their mass spectral data and published data. The first compound (peak 1) was identify as a pelargonidin 3-(6”-p-coumarylglucoside)-5-(4”’-Malonylglucoside) and the second compound was identify as a pelargonidin 3-(6’’-Malonylglucoside). The second one is a major compound that taking up to 91% of the total anthocyanin content in F. padana extract. The antioxidant activity was determined with 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays and results showed that extract possessed high antioxidant capacity.