Byssinosis: Release of prostaglandins, thromboxane, and 5-hydroxytryptamine in bronchopulmonary lavage fluid after inhalation of cotton dust extracts.
Byssinosis: Release of prostaglandins, thromboxane, and 5-hydroxytryptamine in bronchopulmonary lavage fluid after inhalation of cotton dust extracts.
复制标题
棉尘病：吸入棉尘提取物后，支气管肺灌洗液中释放出前列腺素、血栓素和 5-羟色胺。
DOI：
--
复制
发表时间：
1985
影响因子：
6
通讯作者：
S. Ainsworth
中科院分区：
文献类型：
--
作者：
T. Mundie;C. Whitener;S. Ainsworth
将新西兰白色家兔气管内暴露于雾化棉尘提取物（CDE），进行5分钟潮式呼吸，并在暴露后1、4和6小时灌洗15分钟。计数支气管肺泡灌洗液细胞，并测定巨噬细胞和多形核白细胞（PMNs）的数量。细胞募集在暴露于CDE后1小时开始，并在6小时达到平台，由单核细胞和PMN组成。分析灌洗液中前列腺素F2 α（PGF 2 α）、前列腺素E1和E2（PGE）、血栓素B2（TxB 2）和5-羟色胺（5-HT）的浓度。暴露于CDE 4小时后，灌洗液中PGF 2 α、PGE、TxB 2和5-HT最大程度增加。这是第一项研究，以证明在体内释放的花生四烯酸代谢产物和5-羟色胺在肺中响应CDE吸入。该研究还表明，最大介质释放发生在暴露于雾化CDE后4小时。这些发现有力地表明，花生四烯酸代谢产物可完全或部分介导在人急性棉酚反应中观察到的支气管收缩的致病机制。
New Zealand White rabbits were exposed intratracheally to aerosolized cotton dust extract (CDE) for 5 minutes of tidal breathing and lavaged 15 minutes 1, 4, and 6 hours after exposure. Bronchoalveolar lavage cells were counted, and the number of macrophages and polymorphonuclear leukocytes (PMNs) was determined. Cell recruitment, which began 1 hour after exposure to CDE and plateaued at 6 hours, consisted of both mononuclear cells and PMNs. Lavage fluid was analyzed for concentrations of prostaglandin F2 alpha (PGF2 alpha), prostaglandin E1 and E2 (PGE), thromboxane B2 (TxB2), and 5-hydroxytryptamine (5-HT). PGF2 alpha, PGE, TxB2, and 5-HT were maximally increased in the lavage 4 hours after exposure to CDE. This is the first study to demonstrate the in vivo release of arachidonic acid metabolites and 5-HT in the lung in response to CDE inhalation. This study also demonstrates that maximum mediator release occurs at 4 hours after exposure to aerosolized CDE. These findings strongly suggest that arachidonic acid metabolites are available to mediate either totally or partially the pathogenic mechanism(s) of bronchoconstriction seen in the acute byssinotic reaction of man.