Development of microsatellite markers from four Hawaiian corals: Acropora cytherea, Fungia scutaria, Montipora capitata and Porites lobata
Development of microsatellite markers from four Hawaiian corals: Acropora cytherea, Fungia scutaria, Montipora capitata and Porites lobata
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DOI：
10.1007/s12686-009-9118-4
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发表时间：
2010-12-01
影响因子：
1.1
通讯作者：
Toonen, Robert J.
中科院分区：
文献类型：
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作者：
Concepcion, Gregory T.;Polato, Nicholas R.;Toonen, Robert J.
由于缺乏种群遗传标记，对珊瑚群落进行全面的种群遗传研究相对较少。夏威夷群岛为了解这一具有重要生态意义的生物群体的种群遗传结构提供了独特的视角。在这里，我们报告的发展微卫星标记库的holobiont提取物的四种珊瑚：鹿角珊瑚cytherea（n = 50），Fungia scutaria（n = 118），Montipora capitata（n = 140）和Porites lobata（n = 149）。爆炸搜索表明，这些图书馆包含微卫星从珊瑚主机和共生藻内共生体从每个珊瑚。此外，我们还提出了重新设计的核编码区（atps β）的引物，用于M。capitata。我们报告的测试和优化，这些微卫星从A。cytherea和来自M. capitata。使用25个个体，每个物种收集从每个法国护卫舰浅滩（FF）和约翰斯顿环礁（JO），每个位点的等位基因数从2到9不等。A的预期杂合度范围为0.38至0.85和0.08至0.87。cytherea和M.分别为capitata。我们希望这些图书馆将是一个宝贵的资源，并提供额外的有用的微卫星标记的珊瑚主机和虫黄藻。
Comprehensive population genetic studies of coral communities are comparatively rare, because of the scarcity of population genetic markers. The Hawaiian archipelago offers a unique perspective into understanding the population genetic structuring of this ecologically important group of organisms. Here we report the development of microsatellite marker libraries from holobiont extracts of four corals: Acropora cytherea (n = 50), Fungia scutaria (n = 118), Montipora capitata (n = 140) and Porites lobata (n = 149). Blast searches indicate that these libraries contain microsatellites from both the coral host and Symbiodinium endosymbionts from each coral. In addition, we also present redesigned primers for the nuclear coding region (atps beta) for use in M. capitata. We report testing and optimization for seven of these microsatellites from A. cytherea, and eight microsatellites and the atpsb locus from M. capitata. Using 25 individuals per species collected from each French Frigate Shoals (FF) and Johnston Atoll (JO), the number of alleles per locus ranged from 2 to 9. Expected heterozygosities ranged from 0.38 to 0.85 and 0.08 to 0.87 for A. cytherea and M. capitata, respectively. We expect that these libraries will be a valuable resource and provide additional useful microsatellite markers for both the coral host and zooxanthellae.