Data on enrichment of chitosan nanoparticles for intranasal delivery of oligonucleotides to the brain.
Data on enrichment of chitosan nanoparticles for intranasal delivery of oligonucleotides to the brain.
复制标题
用于将寡核苷酸鼻内递送至大脑的壳聚糖纳米粒子富集的数据。
DOI：
10.1016/j.dib.2019.105093
复制
发表时间：
2020
期刊：
影响因子：
1.2
通讯作者：
Sanchez-Ramos,Juan
中科院分区：
文献类型：
--
作者：
Sava,Vasyl;Fihurka,Oksana;Khvorova,Anastasia;Sanchez-Ramos,Juan
数据的制备和表征的壳聚糖为基础的纳米粒子（NP）携带小干扰RNA（siRNA）的非侵入性基因治疗。在稀释的浓度下进行聚电解质络合法以获得相对小（小于200 nm）的NP。为了在鼻内施用的可耐受体积内提供实质剂量的siRNA，使NP经历富集过程。这里提供的NP制造两步法是否包括临时和富集制备？这些制剂之间的差异进行了分析与流体动力学尺寸分布和zeta电位测量。还测试了siRNA亲脂性对NP物理不稳定性的影响。纳米颗粒的生物学评价在我们发表的文章中有描述[1]。
Data on preparation and characterization of chitosan-based nanoparticles (NP) carrying small interfering RNA (siRNA) for non-invasive gene therapy is presented. Polyelectrolyte complexation method was carried out in diluted concentrations to obtain relatively small (less than 200 nm) NP. To provide substantial dose of siRNA within tolerable volume of intranasal administration the NP were subjected to enrichment process. Offered here NP fabrication does two steps process comprise provisional and enriched preparations? The differences between these preparations were analyzed with hydrodynamic size distribution and zeta potential measurements. The effect of siRNA lipophilicity on NP physical instability was also tested. Biological evaluation of nanoparticles is described in our published article [1].