Alterations in lipid peroxidation, antioxidant enzymes, and carcinogen metabolism in liver microsomes of vitamin E-deficient trout and rat.
Alterations in lipid peroxidation, antioxidant enzymes, and carcinogen metabolism in liver microsomes of vitamin E-deficient trout and rat.
复制标题
缺乏维生素 E 的鳟鱼和大鼠肝微粒体中脂质过氧化、抗氧化酶和致癌物代谢的变化。
DOI：
10.1016/0041-008x(92)90147-k
复制
发表时间：
1992
影响因子：
3.8
通讯作者：
Dutchuk,M
中科院分区：
文献类型：
--
作者：
Williams,DE;Carpenter,HM;Buhler,DR;Kelly,JD;Dutchuk,M
用维生素E水平降低70倍的饮食喂养虹鳟鱼16周，导致这些鱼肝微粒体中维生素E水平明显减少（18倍）。缺乏维生素e的鳟鱼体外肝微粒体对脂质过氧化的敏感性以及体内产生的血浆和肝微粒体脂质氢过氧化物水平显著升高。在肝微粒体细胞色素p450依赖的混合功能氧化酶系统或鳟鱼肝微粒体膜的脂肪酸组成中未观察到明显的改变。喂食维生素e缺乏饮食10周的大鼠肝脏微粒体维生素e水平也显著降低。此外，喂食维生素e缺乏饮食的大鼠肝脏总细胞色素P450水平降低（15%），胞质谷胱甘肽水平提高（40%）。缺乏维生素e的鳟鱼肝脏微粒体增强了[3H]-(+)-苯并[a]芘-7,8-二氢二醇与外源DNA的共价结合，这些鱼对这种致癌多环芳烃的急性毒性更加敏感。这些结果表明，鳟鱼可能是研究过氧化途径在致癌过程中的意义以及膳食抗氧化剂对其影响的有用模型。
Feeding rainbow trout for 16 weeks a diet in which the levels of vitamin E were reduced 70-fold resulted in marked depletion (18-fold) of vitamin E levels in liver microsomes from these fish. The susceptibility of hepatic microsomes to lipid peroxidation in vitro and the levels of plasma and liver microsomal lipid hydroperoxides generated in vivo were markedly elevated in vitamin E-depleted trout. No appreciable alterations were observed in the liver microsomal cytochrome P450-dependent mixed-function oxidase system or in the fatty acid composition of trout liver microsomal membranes. Livers from rats fed a vitamin E-deficient diet for 10 weeks also had significantly lower levels of microsomal vitamin E. In addition, total cytochrome P450 levels were depressed (15%) and cytosolic glutathione was enhanced (40%) in livers from rats fed the vitamin E-depleted diet. Covalent binding of [3H]-(+)-benzo[a]pyrene-7,8-dihydrodiol to exogenous DNA in vitro was enhanced with liver microsomes from vitamin E-deficient trout and these fish were much more sensitive to the acute toxicity of this carcinogenic polycyclic aromatic hydrocarbon. These results indicate that trout may be a useful model for studying the significance of peroxidative pathways in carcinogenesis and their manipulation by dietary antioxidants.