Assessment of genetic diversity among nondormant and semidormant alfalfa populations using sequence-related amplified polymorphisms
Assessment of genetic diversity among nondormant and semidormant alfalfa populations using sequence-related amplified polymorphisms
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DOI：
10.2135/cropsci2006.12.0782
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发表时间：
2007-11-01
期刊：
影响因子：
2.3
通讯作者：
Vandemark, George J.
中科院分区：
文献类型：
--
作者：
Ariss, Jennifer J.;Vandemark, George J.
苜蓿（Medicago sativa L.）的合成种群由植物的遗传异质性混合物组成，这可能使分子标记用于检查种群之间的遗传关系变得复杂。分子标记技术与来自多个植物的DNA块相结合，提供了可能是最好的组合的准确性和高通量的表征和苜蓿种群的分化。使用20植物散装DNA模板，我们评估了序列相关扩增多态性（SRAP）将如何估计29个非休眠和半休眠苜蓿种群代表不同的遗传背景和9个历史上公认的美国种质资源之间的遗传相似性。聚类分析的基础上估计的遗传相似性表明，分离的人口在秋季休眠类6至8（半休眠）从秋季休眠类9至11（非休眠），在许多例子中，同一人口的DNA块聚集在一起。一个单一的SRAP标记被确定为只存在于散装的秋眠类6和7，和另一个标记被确定为只检测到散装的秋眠类8。这项研究表明，扩增的SRAP标记从DNA提取的散装植物样品是一种有效的方法，估计遗传相似性内和大量的人口之间。分区域行动方案也可能是有用的间接分类的相对秋季休眠的非特征人口。
Synthetic populations of alfalfa (Medicago sativa L.) consist of genetically heterogeneous mixtures of plants, which may complicate use of molecular markers for examining genetic relationships among populations. Molecular marker techniques combined with bulked DNA from multiple plants provides perhaps the best combination of accuracy and high throughput for characterization and differentiation of alfalfa populations. Using 20-plant bulked DNA templates we assessed how sequence-related amplified polymorphisms (SRAPs) would estimate genetic similarity among 29 nondormant and semidormant alfalfa populations representing diverse genetic backgrounds and the nine historically recognized U.S. germplasm sources. Cluster analysis based on estimates of genetic similarity showed separation of populations in fall dormancy classes 6 to 8 (semidormant) from fall dormancy classes 9 to 11 (nondormant) and in many examples, DNA bulks of the same population clustered together. A single SRAP marker was identified that was present only in bulks of fall dormancy classes 6 and 7, and another marker was identified that was detected only in bulks of fall dormancy class 8. This study demonstrates that the amplification of SRAP markers from DNA extracted from bulked plant samples is an efficient method for estimating genetic similarity both within and between a large numbers of populations. The SRAPs may also be useful for indirectly classifying relative fall dormancies of uncharacterized populations.