Connective tissue growth factor as a major angiogenic agent that is induced by hypoxia in a human breast cancer cell line
Connective tissue growth factor as a major angiogenic agent that is induced by hypoxia in a human breast cancer cell line
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DOI：
10.1016/s0304-3835(01)00683-8
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发表时间：
2001-12-10
期刊：
影响因子：
9.7
通讯作者：
Takigawa, M
中科院分区：
文献类型：
--
作者：
Shimo, T;Kubota, S;Takigawa, M
结缔组织生长因子（CTGF）是一种有效的血管生成因子。在这里，我们提出的证据表明，缺氧诱导的血管生成的人乳腺癌细胞（MDA-231）可以归因于至少部分CTGF，我们的结果表明：（i）CTGF是丰富的存在于MDA-231细胞在体外和体内，（ii）它的分泌是由缺氧上调，（iii）其基因表达在缺氧条件下培养的MDA-231细胞增强。这些数据表明CTGF可能通过旁分泌机制刺激血管生成，从而促进乳腺癌细胞的侵袭。这是第一个证明人类癌细胞在低氧条件下差异表达CTGF蛋白和mRNA的证据。(C)2001爱思唯尔科学爱尔兰有限公司保留所有权利。
Connective tissue growth factor (CTGF) is known to be a potent angiogenic factor. Here, we present the evidence that the hypoxic induction of angiogenesis by human breast cancer cells (MDA-231) can be ascribed at least in part to CTGF, Our results indicate that (i) CTGF is abundantly present in MDA-231 cells in vitro and in vivo, (ii) its secretion is up-regulated by hypoxia, and (iii) its gene expression is enhanced in MDA-231 cells cultured under hypoxic conditions. These data suggest CTGF may stimulate angiogenesis by paracrine mechanisms, thereby contributing to the invasion of breast cancer cells. This is the first evidence that human cancer cells differentially express CTGF protein and mRNA under the control of hypoxic conditions. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.