Control of expression of histocompatibility antigens (H-2) and beta 2-microglobulin in F9 teratocarcinoma stem cells.
Control of expression of histocompatibility antigens (H-2) and beta 2-microglobulin in F9 teratocarcinoma stem cells.
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F9 畸胎癌干细胞中组织相容性抗原 (H-2) 和 β2-微球蛋白表达的控制。
DOI：
10.1073/pnas.78.9.5754
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发表时间：
1981
影响因子：
11.1
通讯作者：
Seidman,JG
中科院分区：
文献类型：
--
作者：
Croce,CM;Linnenbach,A;Huebner,K;Parnes,JR;Margulies,DH;Appella,E;Seidman,JG
与大多数其他细胞类型不同，小鼠畸胎瘤干细胞不表达主要组织相容性抗原。通过印迹杂交，使用克隆的特异于这些mRNA的DNA探针，检测来自F9衍生的畸胎癌干细胞和分化细胞的β 2-微球蛋白和H-2 mRNA的稳态水平。在F9畸胎癌干细胞（克隆12-1）中未检测到H-2或β 2微球蛋白特异性RNA;因此，F9畸胎癌干细胞（克隆12-1）含有的H-2和β 2微球蛋白mRNA不超过分化子细胞（克隆12-1a）的1/10。我们认为，这种主要组织相容性抗原表达的调节是由于主要组织相容性抗原基因，H-2和β 2-微球蛋白的转录控制。这些基因的转录调控伴随着它们的DNase I敏感性的变化。通常，转录失活基因是DNase I抗性的，而活性基因是DNase I敏感的。相反，畸胎瘤干细胞沉默的主要组织相容性抗原基因比分化细胞的活性基因对DNase I更敏感。
Murine teratocarcinoma stem cells, unlike most other cell types, do not express major histocompatibility antigens. The steady-state levels of beta 2-microglobulin and H-2 mRNA from F9-derived teratocarcinoma stem and differentiated cells were examined by blot hybridization using cloned DNA probes specific for these mRNAs. No H-2- or beta 2-microglobulin-specific RNA was detected in F9 teratocarcinoma stem cells (clone 12-1); thus, F9 teratocarcinoma stem cells (clone 12-1) contain no more than 1/10 the H-2 and beta 2-microglobulin mRNAs of the differentiated daughter cells (clone 12-1a). We suggest that this regulation of major histocompatibility antigen expression is due to transcriptional control of the major histocompatibility antigen genes, H-2 and beta 2-microglobulin. The transcriptional regulation of these genes is accompanied by a change in their DNase I sensitivity. Normally, transcriptionally inactive genes are DNase I resistant, while active genes are DNase I sensitive. In contrast, the silent major histocompatibility antigen genes of teratocarcinoma stem cells are more DNase I sensitive than the active genes of the differentiated cells.