INHIBITION OF AFRICAN SWINE FEVER VIRUS-DNA SYNTHESIS BY (S)-9-(3-HYDROXY-2-PHOSPHONYLMETHOXYPROPYL)ADENINE
INHIBITION OF AFRICAN SWINE FEVER VIRUS-DNA SYNTHESIS BY (S)-9-(3-HYDROXY-2-PHOSPHONYLMETHOXYPROPYL)ADENINE
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DOI：
10.1016/0006-291x(88)90644-4
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发表时间：
1988-07-15
影响因子：
3.1
通讯作者：
DECLERCQ, E
中科院分区：
文献类型：
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作者：
ARZUZA, O;GARCIAVILLALON, D;DECLERCQ, E
无环核苷酸类似物（S）-9-（3-羟基-2-膦酰基甲氧基丙基）腺嘌呤[（S）-HPMPA]是一种有效的选择性非洲猪瘟病毒（ASFV）复制抑制剂。用质粒pRPEL-2作探针的DNA-DNA杂交技术，证明（S）-HPMPA对病毒DNA合成有特异性的、剂量依赖性的抑制作用。此外，（S）-HPMPA抑制ASFV感染的MS和Vero细胞中晚期病毒蛋白，特别是IP-73的产生。当在相同的实验条件下进行评价时，膦酰基乙酸（PAA）也会抑制病毒DNA和晚期病毒蛋白质合成，但仅在比（S）-HPMPA所需浓度高10- 20倍的浓度下才会如此。
The acyclic nucleotide analogue (S)-9-(3-hydroxy-2-phosphonylmethoxypropyl)adenine [(S)-HPMPA] is a potent and selective inhibitor of African swine fever virus (ASFV) replication. Using the DNA-DNA hybridization technique with plasmid pRPEL-2 as probe, we have shown that (S)-HPMPA exerts a specific, dose-dependent, inhibitory effect on viral DNA synthesis. Also, (S)-HPMPA inhibits the production of late viral proteins, especially IP-73, in ASFV-infected MS and Vero cells. When evaluated under the same experimental conditions, phosphonoacetic acid (PAA) also caused an inhibition of viral DNA and late viral protein synthesis but only so at a concentration which was 10- to 20-fold higher than that required for (S)-HPMPA.