Identification of proteins associated with ion homeostasis and salt tolerance in barley
Identification of proteins associated with ion homeostasis and salt tolerance in barley
复制标题
大麦中与离子稳态和耐盐性相关的蛋白质的鉴定
DOI：
10.1002/pmic.201300221
复制
发表时间：
2014-06-01
期刊：
影响因子：
3.4
通讯作者：
Zhang, Guoping
中科院分区：
文献类型：
--
作者：
Wu, Dezhi;Shen, Qiufang;Zhang, Guoping
鉴定和鉴定与耐盐相关的蛋白质是揭示其遗传机制的必要条件。本研究以西藏野生大麦XZ 16和著名的耐盐大麦C.A.采用电感耦合等离子体发射光谱仪、二维凝胶电泳和MALDI-TOF/TOF质谱技术对CM 72进行分析，以确定盐诱导的两种基因型之间元素和蛋白质谱的差异。在根和叶中共鉴定出41个差异表达蛋白，它们与离子稳态、细胞氧化还原稳态、代谢过程和光合作用有关。在盐胁迫下，钙调素、Na/K转运蛋白和H+-ATP酶参与了大麦离子稳态的建立。此外，盐胁迫下，核酮糖-1，5-二磷酸羧化酶/加氧酶活化酶和放氧增强子蛋白表达显著上调，表明盐胁迫对光合作用有较大影响。与CM 72相比，XZ 16在盐胁迫下地上部的相对干重较大，Na积累量较低。在盐胁迫下，XZ 16的根中HvNHX 1的表达量增加，一些负责离子稳态和细胞氧化还原稳态的特异性蛋白也被发现。结果表明，西藏野生大麦XZ 16和栽培大麦品种CM 72在耐盐机制上存在差异。
Identification and characterization of proteins involved in salt tolerance are imperative for revealing its genetic mechanisms. In this study, ionic and proteomic responses of a Tibetan wild barley XZ16 and a well-known salt-tolerant barley cv. CM72 were analyzed using inductively coupled plasma-optical emission spectrometer, 2DE, and MALDI-TOF/TOF MS techniques to determine salt-induced differences in element and protein profiles between the two genotypes. In total, 41 differentially expressed proteins were identified in roots and leaves, and they were associated with ion homeostasis, cell redox homeostasis, metabolic process, and photosynthesis. Under salinity stress, calmodulin, Na/K transporters, and H+-ATPases were involved in establishment of ion homeostasis for barley plants. Moreover, ribulose-1,5-bisphosphate carboxylase/oxygenase activase and oxygen-evolving enhancer proteins were significantly upregulated under salinity stress, indicating the great impact of salinity on photosynthesis. In comparison with CM72, XZ16 had greater relative dry weight and lower Na accumulation in the shoots under salinity stress. A higher expression of HvNHX1 in the roots, and some specific proteins responsible for ion homeostasis and cell redox homeostasis, was also found in XZ16 exposed to salt stress. The current results showed that Tibetan wild barley XZ16 and cultivated barley cultivar CM72 differ in the mechanism of salt tolerance.