Immunological characterization of the spike protein of the severe acute respiratory syndrome coronavirus
Immunological characterization of the spike protein of the severe acute respiratory syndrome coronavirus
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DOI：
10.1128/jcm.42.4.1570-1576.2004
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发表时间：
2004-04-01
影响因子：
9.4
通讯作者：
Kwang, J
中科院分区：
文献类型：
--
作者：
Lu, LQ;Manopo, I;Kwang, J
严重急性呼吸综合征(SARS)是由SARS冠状病毒(SARS-CoV)引起的一种新的传染病。SARS-CoV有四种主要结构蛋白，包括核衣壳蛋白、棘蛋白、膜蛋白和小包膜蛋白。在这项研究中，产生了两组截短的Spike蛋白片段，第一组约为210bp的非重叠片段，第二组为750-900bp的重叠片段。从这23个片段中，我们确定了一个259个氨基酸(氨基酸441到700)的片段，它是一个主要的免疫优势表位。该片段得到高效表达，纯化后的片段C能检测到发烧后7~60天采集的33份SARS患者血清样本，但与66份健康人血清样本均无反应。因此，S蛋白C片段被确定为免疫优势抗原，可用于SARS冠状病毒感染的血清学检测。
Severe acute respiratory syndrome (SARS) is a novel infectious disease caused by the SARS-associated coronavirus (SARS-CoV). There are four major structural proteins in the SARS-CoV, including the nucleocapsid, spike, membrane, and small envelope proteins. In this study, two sets of truncated fragments of spike protein were generated, the first were approximately 210-bp nonoverlapping fragments and the second were overlapping segments of 750 to 900 bp. From these 23 fragments, we identified a fragment of 259 amino acids (amino acids 441 to 700) that is a major immunodominant epitope. This fragment was highly expressed, and the purified fragment C could detect all 33 SARS patient serum samples tested, collected from 7 to 60 days after the onset of fever, but had no reactivity with all 66 healthy human serum samples tested. Thus, fragment C of spike protein was identified as an immunodominant antigen and could be used for serological detection of SARS-CoV infection.