Organoids: A historical perspective of thinking in three dimensions.
Organoids: A historical perspective of thinking in three dimensions.
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DOI：
10.1083/jcb.201610056
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发表时间：
2017-01-02
期刊：
影响因子：
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通讯作者：
Bissell MJ
中科院分区：
文献类型：
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作者：
Simian M;Bissell MJ
从这个角度来看，Simian 和 Bissell 讨论了 3D 文化和类器官研究领域迄今为止的演变以及未来的方向。在过去的十年中，出版物的数量急剧增加，其中单个或一组细胞在具有基底膜成分的基质中生长，导致形成被称为类器官的组织样结构。然而，这一研究领域在几十年前就开始了，当时细胞培养的先驱们开始提出我们今天仍在问的问题：器官发生是如何发生的？鉴于我们数万亿个细胞中的基因组序列是相同的，信号如何整合以形成如此截然不同的组织和器官？在这里，我们总结了过去一个世纪的工作如何产生了概念框架，使我们能够在理解组织特异性形态发生程序方面取得进展。事实证明，提供准确且生理相关模型的细胞培养系统的开发是建立适当平台以开发新治疗策略的关键。
In this perspective, Simian and Bissell discuss the evolution of the 3D culture and organoid research field up to now as well as its future directions. In the last ten years, there has been a dramatic surge in the number of publications where single or groups of cells are grown in substrata that have elements of basement membrane leading to the formation of tissue-like structures referred to as organoids. However, this field of research began many decades ago, when the pioneers of cell culture began to ask questions we still ask today: How does organogenesis occur? How do signals integrate to make such vastly different tissues and organs given that the sequence of the genome in our trillions of cells is identical? Here, we summarize how work over the past century generated the conceptual framework that has allowed us to make progress in the understanding of tissue-specific morphogenetic programs. The development of cell culture systems that provide accurate and physiologically relevant models are proving to be key in establishing appropriate platforms for the development of new therapeutic strategies.