HISTOCHEMICAL AND ULTRASTRUCTURAL CHARACTERISTICS OF TUBULAR COMPLEXES IN HUMAN ACUTE-PANCREATITIS
HISTOCHEMICAL AND ULTRASTRUCTURAL CHARACTERISTICS OF TUBULAR COMPLEXES IN HUMAN ACUTE-PANCREATITIS
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DOI：
10.1007/bf01536153
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发表时间：
1989-01-01
影响因子：
3.1
通讯作者：
ADLER, G
中科院分区：
文献类型：
--
作者：
WILLEMER, S;ADLER, G
本文研究了人急性胰腺炎管状小管复合体的形态特征。取自10例急性胰腺炎手术患者的胰腺标本。胰腺酶、角蛋白、肌动蛋白和癌胚抗原的免疫细胞化学与凝集素结合研究和超微结构研究相结合。无论临床症状和胰腺炎持续时间如何，所有患者均可观察到脂肪坏死附近的管状复合体。中间形态的导管状结构以腺泡腔增宽、腺泡细胞高度降低和大的自噬空泡为特征。这些结构结合了所有使用的凝集素，并保留了它们对分泌蛋白的免疫反应性。典型的管状复合体由低立方或扁平的细胞围绕着一个大的腺泡腔组成。他们发现胰腺酶的缺失，L岩藻糖和N-乙酰半乳糖胺的凝集素结合减少，细胞骨架蛋白(角蛋白，肌动蛋白)的增加。结论：人急性胰腺炎小管复合体是一种退化的腺泡细胞，失去了分泌和膜的特性。
The morphologic characteristics of ductlike tubular complexes were studied in human acute pancreatitis. Pancreatic specimens were obtained from 10 patients who were operated on for acute pancreatitis. Immunocytochemistry for pancreatic enzymes, keratin, actin, and carcinoembryonic antigen were combined with lectin-binding studies and ultrastructural investigations. Irrespective of clinical onset and duration of pancreatitis, tubular complexes situated in the vicinity of fat necrosis were observed in all patients. Intermediate forms of ductlike structures were characterized by widening of acinar lumina, decreased height of acinar cells, and large autophagic vacuoles. These structures bound all of the lectins employed and retained their immunoreactivity to secretory proteins. Typical tubular complexes were composed of low cuboidal or flattened cells surrounding a large acinar lumen. They revealed a loss for pancreatic enzymes, a reduced lectin-binding for L-fucose and N-acetylgalactosamine, and an increase for cytoskeletal proteins (keratin, actin). It is concluded that tubular complexes in human acute pancreatitis represent degenerating acinar cells which lost their secretory and membrane characteristics.