IDENTIFICATION OF SOLUBLE FC-RECEPTORS IN MOUSE SERUM AND THE CONDITIONED MEDIUM OF STIMULATED B-CELLS
IDENTIFICATION OF SOLUBLE FC-RECEPTORS IN MOUSE SERUM AND THE CONDITIONED MEDIUM OF STIMULATED B-CELLS
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DOI：
10.1084/jem.160.6.1836
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发表时间：
1984-01-01
影响因子：
15.3
通讯作者：
UNKELESS, JC
中科院分区：
文献类型：
--
作者：
PURE, E;DURIE, CJ;UNKELESS, JC
评估了脂多糖(LPS)激活的鼠脾细胞的表面Fc.gamma.2b/.gamma.1R[对IgG2b和IgG1聚集物特异性的Fc受体]的表达、激活的脾细胞释放的可溶性Fc.gamma.2b/.gamma.1R以及小鼠血清中循环Fc.gamma.2b/.gamma.1R的存在。鼠脾细胞和克隆的 B 细胞系 BCL-1 CW 13.20-3B3 的 LPS 活化导致表面 Fc.gamma.2b/.gamma.1R 增加 5 至 10 倍，并伴随在培养基中出现与 Fc.gamma.2b/.gamma.1R 抗原相关的可溶性分子。 LPS激活后细胞相关和可溶性Fc.gamma.2b/.gamma.1R的增加主要归因于B细胞。还在正常小鼠血清中检测到浓度为 10-9 至 10-8 M 的循环 Fc.gamma.2b/.gamma.1R。循环 Fc.gamma.2b/.gamma.1R 水平随着动物年龄的增长而增加，并且在成年无菌小鼠中较低，而在患有某些自身免疫性疾病的年轻小鼠中升高。循环的Fc.gamma.2b/.gamma.1R与IgG-Sepharose结合，并通过2.4G2 Fab-Sepharose上的亲和层析进行部分纯化。用兔抗Fc.gamma.2b/.gamma.1R血清进行放射性标记和免疫沉淀后，检测到MW 48,000的1种成分。
The expression of surface Fc.gamma.2b/.gamma.1R [Fc receptor specific for IgG2b and IgG1 aggregates] by lipopolysaccharide (LPS)-activated murine spleen cells, the release of soluble Fc.gamma.2b/.gamma.1R by activated spleen cells, and the presence of circulating Fc.gamma.2b/.gamma.1R in mouse serum were evaluated. LPS activation of murine spleen cells and a cloned B cell line, BCL-1 CW 13.20-3B3, resulted in a 5- to 10-fold increase in surface Fc.gamma.2b/.gamma.1R and the concomitant appearance in the culture medium of a soluble molecule that is antigenically related to the Fc.gamma.2b/.gamma.1R. The increase in cell-associated and soluble Fc.gamma.2b/.gamma.1R after LPS activation is attributable primarily to B cells. Circulating Fc.gamma.2b/.gamma.1R was also detected in normal mouse serum at a concentration of 10-9 to 10-8 M. Levels of circulating Fc.gamma.2b/.gamma.1R increased with the age of the animals, and were low in adult germ-free mice and elevated in young mice with certain autoimmune diseases. The circulating Fc.gamma.2b/.gamma.1R bound to IgG-Sepharose, and was partially purified by affinity chromatography on 2.4G2 Fab-Sepharose. After radiolabeling and immunoprecipitation with rabbit anti-Fc.gamma.2b/.gamma.1R serum, 1 component, of MW 48,000, was detected.