Heterogeneity of plant‐associated streptococci as characterized by phenotypic features and restriction analysis of PCR‐amplified 16S rDNA
Heterogeneity of plant‐associated streptococci as characterized by phenotypic features and restriction analysis of PCR‐amplified 16S rDNA
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以表型特征和 PCR 扩增 16S rDNA 限制性分析为特征的植物相关链球菌的异质性
DOI：
10.1046/j.1365-2672.1998.00343.x
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发表时间：
1998
影响因子：
4
通讯作者：
Müller
中科院分区：
文献类型：
--
作者：
Ulrich;Müller
对从牧草中分离的35株链球菌进行了16S rDNA限制性内切酶分析。利用一组7种内切酶，获得了5种16S rDNA基因型。经鉴定，这些菌株属于粪肠球菌Ent.安大略省蒙蒂尔内华达州粪便腰果乳杆菌和乳酸乳球菌。分别为乳酸菌。要将这些分离物归入其中一个物种，用内切酶Hinf I消化就足够了。全细胞蛋白质分析得到的数据与物种鉴定结果一致。此外，发现的五种中有三种被细分为两个相关的亚组。这两种方法都适合于快速和明确地鉴定与植物相关的链球菌。表型性状表现出高度的多样性，在某些情况下与属和种描述中通常给出的预期特征相反。结果表明，与它们高度的表型异质性相比，所确定的物种具有密切的亲缘关系。
Thirty‐five strains of streptococci isolated from forage grasses were examined by restriction analysis of PCR‐amplified 16S rDNA. Using a set of seven endonucleases, five 16S rDNA genotypes were obtained. The isolates could be identified as belonging to the species Enterococcus faecium, Ent. mundtii, Ent. faecalis, Ent. casseliflavus and Lactococcus lactis ssp. lactis, respectively. To assign the isolates to one of these species, digestion with the endonuclease Hinf I was sufficient. Data obtained from whole‐cell protein analysis were in accordance with the species identification. Additionally, three of the five species found were subdivided into two related subgroups. Both methods are suitable for a rapid and unequivocal identification of plant‐associated streptococci. The phenotypic characters showed a high diversity and were in some cases contrary to the expected features usually given in genus and species descriptions. The results demonstrated the close relatedness of the determined species in contrast to their high phenotypic heterogeneity.