Development of a 96-well plate iodine binding assay for amylose content determination
Development of a 96-well plate iodine binding assay for amylose content determination
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DOI：
10.1016/j.carbpol.2014.09.015
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发表时间：
2015-01-22
影响因子：
11.2
通讯作者：
Shi, Y. -C.
中科院分区：
文献类型：
--
作者：
Kaufman, R. C.;Wilson, J. D.;Shi, Y. -C.
谷物淀粉直链淀粉/支链淀粉 (AM/AP) 对于食品和工业应用的功能特性至关重要。传统的 AM/AP 方法既耗时又费力，因此很难筛选评估育种样本和调查环境对淀粉发育的影响所需的大样本集。目的是调整和优化 96 孔板形式的碘结合测定，以在 lambda 620 nm 和 lambda 510 nm 下进行测量。将直链淀粉含量的标准曲线缩放至 96 孔板格式，并证明单波长和双波长的 R-2 值分别为 0.999 和 0.993。平板法适用于大范围的直链淀粉含量：高直链玉米淀粉为 61.7 +/- 2.3%，普通小麦淀粉为 29.0 +/- 0.74%，蜡质玉米淀粉为 1.2 +/- 0.9%。对于普通型淀粉，该方法显示出比 Concanavalin A 方法稍高的直链淀粉含量值；但与比色杯规模的碘结合测定一致。由爱思唯尔有限公司出版
Cereal starch amylose/amylopectin (AM/AP) is critical in functional properties for food and industrial applications. Conventional methods of AM/AP are time consuming and labor intensive making it difficult to screen the large sample sets necessary for evaluating breeding samples and investigating environmental impact on starch development. The objective was to adapt and optimize the iodine binding assay in a 96-well plate format for measurement at both lambda 620 nm and lambda 510 nm. The standard curve for amylose content was scaled to a 96-well plate format and demonstrated R-2 values of 0.999 and 0.993 for single and dual wavelengths, respectively. The plate methods were applicable over large ranges of amylose contents: high amylose maize starch at 61.7 +/- 2.3%, normal wheat starch at 29.0 +/- 0.74%, and a waxy maize starch at 1.2 +/- 0.9%. The method exhibited slightly greater amylose content values than the Concanavalin A method for normal type starches; but is consistent with cuvette scale iodine binding assays. Published by Elsevier Ltd.