IODINE SUPPRESSION OF IODIDE UPTAKE IN FRTL-5 THYROID-CELLS
IODINE SUPPRESSION OF IODIDE UPTAKE IN FRTL-5 THYROID-CELLS
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DOI：
10.1210/endo-118-6-2477
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发表时间：
1986-06-01
期刊：
影响因子：
4.8
通讯作者：
SANTISTEBAN, P
中科院分区：
文献类型：
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作者：
GROLLMAN, EF;SMOLAR, A;SANTISTEBAN, P
FRTL-5细胞暴露于超过3微米的碘(I-)可抑制对I-的集中摄取。在稳态下测得的I-摄取抑制是由于I-内流速率的降低，而不是由于I-外流的影响。暴露于NaI与T4分泌减少和Na+依赖的氨基酸积累减少有关。I-和氨基酸转运的抑制随着暴露时间和I-使用浓度的增加而成比例增加，但与FRTL-5细胞cAMP水平的变化无关。然而，在与NaI孵育期间，当他巴唑存在时，抑制I-的作用被阻断。与体内研究一致，FRTL-5细胞中的i-抑制似乎依赖于有机化过程中的中间体，并且不依赖于TSH诱导的cAMP介导的作用。
Exposure of FRTL-5 cells to iodide (I-) in excess of 3 .mu.M suppresses the concentrative uptake of I-. The depression of I- uptake measured at the steady state is due to a decrease in the rate of I- influx and not to an effect on I- efflux. Exposure to NaI is associated with decreased T4 secretion and also depressed Na+-dependent amino acid accumulation. The depression in I- and amino acid transports increases proportionately with the duration of exposure and concentration of I- used but is not associated with alterations in FRTL-5 cell cAMP levels. The I- suppression effect is blocked, however, when methimazole is present during the incubation with NaI. In agreement with studies in vivo, I- suppression in FRTL-5 cells appears to depend on an intermediate in the organification process and to be independent of a TSH-induced cAMP-mediated action.