Laboratory rearing technique of Mahanarva fimbriolata (Stål) (Hemiptera: Cercopidae)
Laboratory rearing technique of Mahanarva fimbriolata (Stål) (Hemiptera: Cercopidae)
复制标题
DOI：
10.1590/s0103-90162007000100011
复制
发表时间：
2007-02
影响因子：
2.6
通讯作者：
J. F. García;P. Botelho;J. Parra
中科院分区：
文献类型：
--
作者：
J. F. García;P. Botelho;J. Parra
由于在甘蔗和牧草中作为害虫的尾螨的重要性，已经进行了研究以解决与这些昆虫的生物生态学和控制相关的方面。本研究旨在发展甘蔗上毛额真夜蛾Mahanarva fimbriolata（Stal）（半翅目：瘿蚊科）的饲养技术。将成虫隔离在含有一株甘蔗植物的圆柱形笼中，植物基部周围有湿润的棉花盘作为产卵部位。通过在流动水中在筛子上洗涤将卵从棉盘中取出，并保持在湿滤纸上的陪替氏培养皿中，直至卵孵化。将新孵化的甘蔗转移到甘蔗根部，并放入带盖的盒子中，以保持潮湿和黑暗的环境，有利于它们的发育和维持根系，为发育中的甘蔗提供食物。该方法允许M.在实验室条件下全年保持fimbriolata，这肯定会促进对这种昆虫的研究。
Due to the importance of cercopids as pests in sugarcane and forage grass, researches have been conducted in order to address aspects related to the bioecology and control of these insects. The objective of this study was to develop a rearing technique for Mahanarva fimbriolata (Stal) (Hemiptera: Cercopidae) on sugarcane, in the laboratory (25 ± 1oC; 70 ± 10% RH; and 14-hour photophase). Adults were isolated in cylindrical cages containing one sugarcane plant, with a moistened cotton disc surrounding the base of the plant as a site for oviposition. Eggs were removed from the cotton disc by washing it in running water over sieves and maintained in Petri dishes on moistened filter paper until the nymphs hatched. The newly hatched nymphs were transferred to sugarcane roots and placed into boxes with lids, to keep a moistened and dark environment favoring their development and maintaining the root system, providing food access to the developing nymphs. This method allowed M. fimbriolata to be maintained in laboratory conditions throughout the year, which would certainly foster research on this insect.