Expression of the fructose receptor BmGr9 and its involvement in the promotion of feeding, suggested by its co-expression with neuropeptide F1 in Bombyx mori.
Expression of the fructose receptor BmGr9 and its involvement in the promotion of feeding, suggested by its co-expression with neuropeptide F1 in Bombyx mori.
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DOI：
10.1016/j.ibmb.2016.06.001
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发表时间：
2016-08
影响因子：
3.8
通讯作者：
Dingze Mang;Min-Ji Shu;Shiho Tanaka;S. Nagata;Tomoyuki Takada;Haruka Endo;Shingo Kikuta;H. Tabunoki;K. Iwabuchi;R. Sato
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文献类型：
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作者：
Dingze Mang;Min-Ji Shu;Shiho Tanaka;S. Nagata;Tomoyuki Takada;Haruka Endo;Shingo Kikuta;H. Tabunoki;K. Iwabuchi;R. Sato
昆虫味觉受体（Insect gustatory receptors，Grs）是一个具有7个跨膜结构域的蛋白质大家族的成员，它为昆虫提供了检测对取食、交配和产卵至关重要的化学信号的能力。迄今为止，已经通过基因组研究鉴定了69个BmGrs基因。BmGr9已被证明对果糖有特异性反应，并作为果糖选择性激活的配体门控离子通道发挥作用。然而，该Gr表达的位点仍不清楚。我们用逆转录（RT）-PCR证明BmGr9广泛表达于中枢神经系统（CNS），以及口腔感觉器官。此外，使用抗BmGr9抗血清进行免疫组织化学，以显示BmGr9在口腔感觉器官的细胞中表达，包括上颌帽状突、上颌须、唇和唇，以及在CNS的推定神经分泌细胞中表达。此外，双重免疫组化分析表明，大多数BmGr9表达细胞与脑中假定的神经肽F1表达细胞共定位，表明BmGr9参与促进摄食行为。此外，大脑中一部分表达BmGr9的细胞与表达BmGr6的细胞共定位，BmGr6是糖受体进化枝的一种分子，这表明果糖以外的糖参与了家蚕摄食行为的调节。
Insect gustatory receptors (Grs) are members of a large family of proteins with seven transmembrane domains that provide insects with the ability to detect chemical signals critical for feeding, mating, and oviposition. To date, 69Bombyx moriGrs (BmGrs) genes have been identified via genome studies. BmGr9 has been shown to respond specifically to fructose and to function as a ligand-gated ion channel selectively activated by fructose. However, the sites where this Gr are expressed remain unclear. We demonstrated using reverse transcription (RT)-PCR thatBmGr9is widely expressed in the central nervous system (CNS), as well as oral sensory organs. Additionally, immunohistochemistry was performed using anti-BmGr9 antiserum to show that BmGr9 is expressed in cells of the oral sensory organs, including the maxillary galea, maxillary palps, labrum, and labium, as well as in putative neurosecretory cells of the CNS. Furthermore, double immunohistochemical analysis showed that most BmGr9-expressing cells co-localized with putative neuropeptide F1-expressing cells in the brain, suggesting that BmGr9 is involved in the promotion of feeding behaviors. In addition, a portion of BmGr9-expressing cells in the brain co-localized with cells expressing BmGr6, a molecule of the sugar receptor clade, suggesting that sugars other than fructose are involved in the regulation of feeding behaviors inB. morilarvae.