POLYMERASE CHAIN-REACTION TO DETECT HUMAN CYTOMEGALOVIRUS IN LIVERS OF INFANTS WITH NEONATAL HEPATITIS
POLYMERASE CHAIN-REACTION TO DETECT HUMAN CYTOMEGALOVIRUS IN LIVERS OF INFANTS WITH NEONATAL HEPATITIS
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DOI：
10.1016/0016-5085(92)90038-z
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发表时间：
1992-09-01
期刊：
影响因子：
29.4
通讯作者：
LEE, CY
中科院分区：
文献类型：
--
作者：
CHANG, MH;HUANG, HH;LEE, CY
根据血清学及尿培养研究，台湾新生儿肝炎与人类巨细胞病毒感染密切相关。为了获得更直接的证据，进一步将巨细胞病毒与新生儿肝炎的发病机制联系起来，采用聚合酶链反应（PCR）对50例新生儿肝炎婴儿的肝组织中的巨细胞病毒基因组进行了研究。26例胆道闭锁婴儿和另外30例诊断为新生儿肝炎、胆汁淤积或肝炎以外的婴儿和儿童的肝组织也进行了比较研究。来自立即早期基因1和2区域的序列用作引物。50例新生儿肝炎患儿肝组织中23例（46%）巨细胞病毒基因组阳性，26例胆道闭锁患儿肝组织中2例阳性，30例非新生儿肝炎患儿肝组织中无一例阳性。PCR检测结果与血清学和尿培养结果有良好的相关性。本研究为巨细胞病毒在新生儿肝炎发病机制中的作用提供了进一步的证据。
Neonatal hepatitis is closely related to human cytomegalovirus infection in Taiwan, a conclusion based on serological and urine culture studies. To obtain more direct evidence further relating cytomegalovirus to the pathogenesis of neonatal hepatitis, the cytomegalovirus genome was studied in the liver tissues of 50 infants with neonatal hepatitis using the polymerase chain reaction (PCR). Liver tissues from 26 infants with biliary atresia and another 30 infants and children with diagnoses other than neonatal hepatitis, cholestasis, or hepatitis were also studied for comparison. Sequences from the immediate early gene 1 and 2 regions were used as primers. The liver tissues from 23 (46%) of the 50 infants with neonatal hepatitis were positive for cytomegalovirus genome, whereas those of 2 of the 26 infants with biliary atresia and none of the liver tissues from 30 infants and children without neonatal hepatitis were positive for cytomegalovirus genome, by PCR. The results of PCR correlated well with that of serology and urine culture. This study provides further evidence of cytomegalovirus in the pathogenesis of neonatal hepatitis.