Direct interaction between adenovirus E1A protein and the TATA box binding transcription factor IID.
Direct interaction between adenovirus E1A protein and the TATA box binding transcription factor IID.
复制标题
腺病毒E1A蛋白与TATA盒结合转录因子IID之间的直接相互作用。
DOI：
10.1073/pnas.88.12.5124
复制
发表时间：
1991
影响因子：
11.1
通讯作者：
Weinmann,R
中科院分区：
文献类型：
--
作者：
Horikoshi,N;Maguire,K;Kralli,A;Maldonado,E;Reinberg,D;Weinmann,R
腺病毒E1 A长期以来已知激活/抑制细胞和病毒转录。在固定化E1 A蛋白柱上层析后，核提取物的转录活性被耗尽，所述固定化E1 A蛋白柱特异性地保留转录因子（TF）IID。E1 A和人TFIID之间的直接相互作用比E1 A和酵母TFIID之间的直接相互作用更强，这表明可能涉及人类蛋白质的独特序列。我们已经证明，这种相互作用直接发生在细菌产生的E1 A和细菌产生的人TFIID在蛋白质印迹分析。我们认为E1 A蛋白可能通过与TFIID接触，将调控信号从上游激活因子传递到转录机制的基础元件。
Adenovirus E1A has long been known to activate/repress cellular and viral transcription. The transcriptional activity of nuclear extracts was depleted after chromatography on immobilized E1A protein columns that specifically retained the transcription factor (TF) IID. Stronger direct interactions between E1A and human TFIID than between E1A and yeast TFIID suggest that the unique sequences of the human protein may be involved. We have demonstrated that this interaction occurs directly between bacterially produced E1A and bacterially produced human TFIID in a protein blot assay. We propose that E1A protein may transduce regulatory signals from upstream activators to basal elements of the transcriptional machinery by contacting TFIID.